The influence of mechanical pressure on the migration of dural cells in different parts and their ability to promote bone formation
10.19405/j.cnki.issn1000–1492.2026.09.009
- VernacularTitle:机械压力对不同部位硬脑膜细胞迁移及促成骨能力的影响
- Author:
Haidong ZHANG
1
;
Jie SHEN
1
;
Yajie GAO
2
;
Guo SHAO
3
;
Chunyang ZHANG
1
;
Zhijun ZHAO
1
Author Information
1. Baotou Medical College， Inner Mongolia University of Science and Technology， Baotou 014010
2. Institute of Neurosurgical Diseases， Baotou Medical College， Baotou 014010
3. Translational Medicine Center， The Third People's Hospital of Longgang District， Shenzhen 518112
- Publication Type:Journal Article
- Keywords:
unmature dura mater cells;
cranial suture mesenchymal stem cells;
mechanical pressure;
osteogenic differentiation;
migration ability
- From:
Acta Universitatis Medicinalis Anhui
2026;61(9):1576-1583
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of mechanical pressure regulation on the migration and bone-promoting ability of suture dural cells （S-DCs） and non-suture dural cells （NS-DCs）. MethodsDural cells （S-DCs）， non-cranial suture dural cells （NS-DCs） and cranial suture mesenchymal stem cells （CS-MSCs） of 5-day-old SD rats were isolated and cultured. Four experimental conditions， namely S-DCs-Normal， S-DCs-Stress， NS-DCs-Normal and NS-DCs-Stress， were set according to the source of the cells and whether mechanical pressure （1.07 kPa， 24 hours） was applied. The migration ability of dural cells was detected by cell scratch test. Four experimental condition culture media were collected and co-cultured with CS-MSCs for osteogenic induction. Calcium salt deposition was detected by alizarin red staining， and the expression of osteogenesis-related proteins was detected by Western blot. ResultsCompared with the Normal group， the migration abilities of both S-DCs and NS-DCs decreased after Stress treatment （P<0.05）. Compared with S-DCs， NS-DCs has a weaker migration ability under the same processing conditions （P<0.05）. The conditioned medium experiment showed that Stress treatment reduced the osteogenic ability of CS-MSCs （P<0.05）， and the osteogenic effect of the conditioned medium derived from NS-DCs was weaker than that of S-DCs （P<0.05）. The Western blot results were consistent with the trend of alizarin red staining. Both Stress treatment and NS-DCs sources could down-regulate the expression of osteogenesis-related proteins （P<0.05）. ConclusionMechanical stress can significantly inhibit the migration of dural cells and the osteogenic differentiation process of CS-MSCs mediated by it. The mechanical tolerance and osteogenic regulation ability of S-DCs are significantly better than those of NS-DCs.
