Effects of platelet-rich fibrin extract on injured endometrial stromal cells induced by mifepristone and its mechanism study
10.19405/j.cnki.issn1000-1492.2026.08.005
- VernacularTitle:富血小板纤维蛋白提取液对米非司酮诱导损伤的子宫内膜间质细胞的修复作用及其机制研究
- Author:
Ping QIU
1
;
Jing TANG
1
;
Yu LIU
1
;
Hui LIU
1
;
Yaqing LIU
1
;
Renjie CHEN
1
;
Jun SHEN
2
;
Liwen CHEN
1
Author Information
1. Department of Clinical Laboratory Diagnostics Research， The Second Clinical College of Anhui Medical University， Hefei 230601
2. Department of Neurosurgery， The First Affiliated Hospital of Wannan Medical University， Wuhu 241001
- Publication Type:Journal Article
- Keywords:
PRF;
growth factors;
endometrial injury;
cell proliferation;
cellular repair;
release kinetics
- From:
Acta Universitatis Medicinalis Anhui
2026;61(8):1364-1374
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the biological reparative effects of platelet-rich fibrin （PRF） extract on injured endometrial stromal cells （ESCs） induced by mifepristone and the underlying molecular mechanisms. MethodsAn ESCs injury model was established using mifepristone treatment. Cells were divided into four groups： untreated control （Con）， mifepristone-injured group （M）， mifepristone treatment+PRF extract group （M+P）， and mifepristone treatment+PRF extract+growth factor inhibitors treatment group， including M+P+T， M+P+Me， and M+P+CJ. Cell viability was detected using the CCK-8 assay， and migration ability was evaluated via the scratch wound healing assay. The release kinetics of growth factors， including vascular endothelial growth factor （VEGF）， platelet-derived growth factor-BB （PDGF-BB）， and transforming growth factor-beta 1 （TGF-β1） from PRF extract were measured by ELISA. The mRNA and protein expression levels of apoptosis-related factors （Bcl-2， Bax， and Caspase-3） were analyzed by RT-qPCR and Western blot， respectively. ResultsVEGF， PDGF-BB， and TGF-β1 were continuously released from the PRF extract over two weeks， with peak release observed on days 3-7. The extract significantly restored the proliferation and migration of mifepristone-injured ESCs （all P<0.000 1）. PRF extract up-regulated the mRNA and protein expression of the anti-apoptotic factor Bcl-2 （all P<0.000 1） and down-regulated that of the pro-apoptotic factors Bax and Caspase-3 （all P<0.000 1） in mifepristone-injured ESCs. Inhibitors of VEGF， PDGF-BB， and TGF-β1 （Tanshinone IIA， Methylnissolin， and CJJ300） significantly reversed the protective effects of PRF extract on mifepristone-injured ESCs （all P<0.05）. ConclusionPRF extract promotes the repair of injured ESCs by enhancing cell proliferation， attenuating apoptosis， and increasing migration capacity， which involve multiple growth factors， including VEGF， PDGF-BB， and TGF-β1.
