Analysis of differential interactions between SARS-CoV-NP and SARS-CoV-2-NP with host proteins
10.19405/j.cnki.issn1000-1492.2026.08.003
- VernacularTitle:SARS-CoV与SARS-CoV-2 NP蛋白同宿主蛋白互作差异的分析
- Author:
Xinyi LI
1
;
Lüyin SUN
1
;
Zhuping MA
1
;
Yonggang LI
2
;
Xiaoli TAO
1
Author Information
1. Department of Pathogenic Biology， School of Basic Medical Sciences， Jinzhou Medical University， Jinzhou 121000
2. Science Experiment Center， Shenyang Medical College， Shenyang 110000
- Publication Type:Journal Article
- Keywords:
novel coronavirus;
severe acute respiratory syndrome coronavirus;
differential proteome;
NP;
protein interaction;
bioinformatics
- From:
Acta Universitatis Medicinalis Anhui
2026;61(8):1348-1354
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the differences in the interactions with host proteins between severe acute respiratory syndrome coronavirus （SARS-CoV） nucleocapsid protein （NP） and SARS-CoV-2-NP， and to screen out specific differential proteins for analyzing the pathogenicity differences between the two at the protein level. MethodsAll proteins in Vero cells interacting with SARS-CoV-NP or SARS-CoV-2-NP were screened by the pull-down assay， and the screened proteins were analyzed by mass spectrometry for quantitative and qualitative analysis， protein function annotation， and protein-protein interaction （PPI） network construction by mass spectrometry. ResultsA total of 1 345 proteins were retrieved from the database. Compared with the SARS-CoV-2-NP group， the SARS-CoV-NP group had 358 downregulated differentially expressed proteins and 476 upregulated differentially expressed proteins. Biosignature analysis revealed that these proteins were predominantly located in the cytoplasm and nucleus. They participated in biological processes such as regulation of biological processes and metabolic processes. Their molecular functions included ion-binding， nucleotide-binding， and carbohydrate derivative-binding. These proteins were closely associated with diseases linked to metabolism and genetic information processing. Many of them contained functional structural domains like RNA recognition motifs and tryptophan-aspartic acid repeat （WD） domains. ConclusionCompared with the SARS-CoV-NP group， the SARS-CoV-2-NP group exhibited multiple differential host proteins， providing a basis for investigating the molecular pathogenesis and therapeutic targets of SARS-CoV-2.
