Pharmacokinetic study of flumazenil sublingual spray in beagle dogs
10.12206/j.issn.2097-2024.202503042
- VernacularTitle:氟马西尼舌下喷雾剂在比格犬体内的药动学研究
- Author:
Junjie HUA
1
;
Min ZHU
1
;
Yingnan ZHANG
1
;
Guangzhao LU
1
;
He ZHANG
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Publication Type:Researchreports
- Keywords:
flumazenil;
sublingual spray;
beagle dogs;
pharmacokinetics
- From:
Journal of Pharmaceutical Practice and Service
2026;44(9):483-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pharmacokinetics of flumazenil sublingual spray in Beagle dogs. Methods Twelve Beagle dogs were grouped in a two-period, two-sequence crossover study design, receiving intravenous injection or sublingual spray administration. Blood samples （2 ml） were collected from the leg vein at 0.16, 0.33, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0, 2.5, 3.0, 4.0, 6.0 and 8.0 h post-dosing. The plasma concentration of flumazenil was quantified by liquid chromatography-tandem mass spectrometry （LC-MS/MS） with an internal standard method, and key pharmacokinetic parameters were calculated. Results The tmax values for flumazenil injection and sublingual spray were 0.17 h and （0.28±0.10） h, respectively; cmax values were （8.14±0.81） and （10.41±1.72） ng/ml; and AUC0–∞ values were （6.90±0.99） and （10.97±1.68）（ng·h）/ml. The bioavailability of the sublingual spray was （80.79±0.14）%. Conclusion Flumazenil sublingual spray demonstrated rapid absorption and high bioavailability in Beagle dogs.
