Determination of gallic acid in sicaokeyin gel by HPLC method
10.12206/j.issn.2097-2024.202508053
- VernacularTitle:HPLC法测定四草克银凝胶中没食子酸的含量
- Author:
Lianyan YANG
1
;
Weirong XIAO
1
;
Shiyu WEN
1
;
Xinxin XIE
2
;
Hongmin ZHANG
2
;
Bin CHEN
1
Author Information
1. Shanghai Jing’an Institute for Food and Drug Control, Shanghai 200435, China.
2. Shanghai Skin Disease Hospital, Shanghai 200443, China.
- Publication Type:Originalarticles
- Keywords:
Sicaokeyin gel;
sanguisorba officinalis;
gallic acid;
content assay;
HPLC method
- From:
Journal of Pharmaceutical Practice and Service
2026;44(9):468-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective Sanguisorba officinalis is the principal medicinal ingredient of Sicaokeyin gel. Gallic acid is commonly used as a key indicator component for evaluating the quality of Sanguisorba officinalis and its preparations. To establish a HPLC method for the determinant of gallic acid in Sicaokeyin gel. Methods With gallic acid as reference substance，HPLC analysis was performed on a Agilent Eclipse XDB-C18 column （4.6 mm ×25 mm，5 μm） with methanol-0.05%phosphoric acid （5∶95） under isocratic elution. The flow rate was 1.0 ml/min and the detection wavelength was 272 nm. The column temperature was 30℃, the injection volume was 10 μl. Results Gallic acid showed good linear relationship in the range of 1.004-50.18 μg/ml （r=0.999 9）. The average recovery was 102.9% （n=9）. Conclusion The method was easy to operate; the result was accurate and reliable, which could be used for the quality control of sanguisorba officinalis in Sicaokeyin gel.
