Effects of Gurgem-13 fractions on the viability of hepatic stellate cells
- VernacularTitle:Гүргэм-13 эмийн фракцуудын элэгний одлог эсийн амьдрах чадварт үзүүлэх нөлөөг судалсан дүн
- Author:
Suodunfu
1
;
Mingming Bao
2
;
Suolonggaowa
3
;
Molor-Erdene P
1
;
Tserendagva D
4
Author Information
1. Department of Internal Medicine, ISMM, MNUMS
2. Inner Mongolia Medical University, China
3. Inner Mongolia International Mongolian Hospital, China
4. Mongolian National University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Gurgem-13;
Liver stellate cells;
Proliferation
- From:
Mongolian Journal of Health Sciences
2026;95(5):72-76
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Gurgem-13, a traditional Mongolian medicinal formula, is described in the Four Medical Tantras as having therapeutic effects against liver disorders, toxins, renal “wind” conditions, urinary retention, hot-natured conditions, and parasitic infections. It has been widely used in Mongolian traditional medical practice for the treatment of various liver diseases.
Aim:To evaluate the effects of Gurgem-13 fractions on the viability of LX-2 cells (human hepatic stellate cell line).
Materials and Methods:Gurgem-13 powder was extracted by multiple solvents, including acetonitrile, methanol, distilled water, petroleum ether, n-hexane, ethyl acetate, and anhydrous ethanol, to obtain distinct fractions. LX-2 cells were cultured under standard conditions and treated with varying concentrations of each fraction. Cell proliferation was assessed using the MTT assay.
Results:All Gurgem-13 fractions exhibited dose-dependent inhibitory effects on LX-2 cell proliferation. At concentrations of 50 and 100 µL/mL, a statistically significant reduction in cell proliferation was observed (p<0.05). Among the tested fractions, the ethyl acetate fraction demonstrated the highest inhibitory activity.
Conclusion:Gurgem-13 fractions, particularly the ethyl acetate fraction, significantly suppresses the proliferation of hepatic stellate cells in vitro, suggesting potential antifibrotic activity. These findings support the traditional use of Gurgem-13 in liver disease and suggest its potential therapeutic value against liver fibrosis.
- Full text:2026092722554269015Гүргэм-13 эмийн фракцуудын.pdf
