Results of the study on antioxidant activity of selected polyherbal teas for hepatobiliary support
- VernacularTitle:Элэг, цөсний үйл ажиллагааг дэмжих үйлдэлтэй нийлмэл найрлагатай зарим зэхмэл цайны антиоксидант идэвхийг судалсан үр дүн
- Author:
Tungalag B
1
;
2
;
Batkhuyag P
3
;
Enkh-Amgalan B
2
;
Purevdorj E
4
;
Khaliun N
1
Author Information
1. Department of Pharmacology, School of Bio-Medicine, MNUMS
2. Department of Clinical Medicine, Darkhan Medical School, MNUMS
3. School of Pharmacy, MNUMS
4. Laboratory of Natural Product Chemistry, Institute of Chemistry and Chemical Technology, MAS
- Publication Type:Journal Article
- Keywords:
Polyherbal tea;
Antioxidant activity;
Phenolic compounds;
Flavonoids;
DPPH;
ABTS⁺;
FRAP
- From:
Mongolian Journal of Health Sciences
2026;92(2):159-164
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Herbal teas are rich in bioactive compounds with antioxidant, anti-inflammatory, and hepatobiliary supportive properties. Despite their long-standing use in Mongolian traditional medicine for liver and gallbladder health, comprehensive scientific data regarding the specific chemical composition and antioxidant activities of these polyherbal formulations remain limited.
Aim:To evaluate the bioactive compound content and antioxidant activity of a polyherbal tea formulation for supporting liver and gallbladder function, with a focus on the influence of solvent concentration.
Materials and Methods:The study utilized a polyherbal tea consisting of five plant species: Achillea asiatica Serg., Thalictrum foetidum L., Zygophyllum potaninii Maxim., Tanacetum vulgare L., and Rheum undulatum L. Samples were extracted using 30% and 70% ethanol. Preliminary phytochemical screening was performed using thin-layer chromatography (TLC). Total phenolic content (TPC) was determined using the Folin-Ciocalteu method, and total flavonoid content (TFC) was measured via the AlCl3 colorimetric assay. Antioxidant capacity was assessed through DPPH, ABTS+ and FRAP assays.
Results:Phytochemical screening identified flavonoid derivatives, glycosides, and terpenoids in both extracts, while alkaloids were not detected. Quantitative analysis showed that the 70% ethanolic extract contained significantly higher levels of total phenolics (5.518±0.24%) and total flavonoids (1.513±0.023) compared to the 30% extract (p < 0.05). The 70% extract also demonstrated superior antioxidant potency, with lower (IC50) values for DPPH (55.37 µg/mL) and ABTS (86.08 µg/mL). Additionally, the FRAP assay confirmed the 70% extract had substantially higher reducing power (1440.84 µM Fe²⁺/L).
Conclusions:1. Phytochemical analysis confirmed that the polyherbal tea is primarily composed of flavonoid derivatives, glycosides, and terpenoids. The total phenolic and flavonoid contents in the 70% ethanolic extract were significantly higher (p < 0.05) than those in the 30% extract. 2. The free radical scavenging activity evaluated by DPPH (IC50) 55.37 µg/mL) and ABTS (IC50) 86.08 µg/mL) assays indicated that the 70% ethanolic extract possesses markedly stronger antioxidant activity compared to the 30% extract. 3. The iron ion reducing power (FRAP: 1440.84 µM/L) showed a concentration-dependent linear relationship. The positive correlation between polyphenol content and antioxidant activity confirms that this herbal formulation has a scientific basis for its traditional use in supporting liver and gallbladder function.
- Full text:202609272048416888530.pdf
