Results of a comparative study on the effects of a mixture of bacterial metabolites and aloe vera (Aloe barbadensis Miller) on burn wound healing
- VernacularTitle:Бактерийн метаболит ба зуун настын (Aloe barbadensis Miller) холимгийн түлэнхийн шархны эдгэрэлтэд үзүүлэх нөлөөг харьцуулан судалсан дүн
- Author:
Nyam-Erdene N
1
;
Sevjidmaa B
2
;
Manaljav B
3
;
Munkhtushig O
4
;
Barsbold M
4
;
Otgonsuren B
4
;
Arvinzaya B
4
;
Uugangerel E
5
;
6
;
Enkhmaa D
5
;
6
;
Tsevelmaa N
1
;
5
;
Nandin-Erdene M
5
;
7
;
Suvdaa B
5
;
Battogtokh Ch
5
;
Khongorzul B
1
;
5
Author Information
1. Academic Research Center, Graduate School, MNUMS
2. Department of Microbiology and Infection Prevention and Control, School of Biomedicine, MNUMS
3. School of Pharmacy, MNUMS
4. School of Medicine, MNUMS
5. Institute of Biomedical Sciences, MNUMS
6. Department of Medical Chemistry, School of Biomedicine, MNUMS
7. Department of Biochemistry, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Skin commensal bacteria, Burn injury, Wound healing dynamics
- From:
Mongolian Journal of Health Sciences
2026;91(1):32-37
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Skin wounds can occur for many reasons, including UV radiation, mechanical burns, trauma, and chronic
inflammation caused by metabolic factors. In Mongolia, as of 2022, household burns were the top 5 causes of injuries, the
leading cause of injuries in children aged 0-5, and accounted for 18.4% of all illnesses in children aged 0-5. Skin burns
and wounds take a long time to heal, which causes emotional, aesthetic, and economic stress for doctors. Developing in
novative, effective, and low-side effects therapeutic products to improve wound healing is of great clinical and economic
importance. The lack of research on the effects of bacterial metabolites and aloe vera (Aloe barbadensis Miller) on burn
wounds was the basis for our research.
Aim:To determine the effects of a mixture of bacterial metabolites and aloe vera on burn wounds in experimental animals.
Materials and Methods: Eight-week-old BALB/c mice were divided into three groups: control group, bacterial metabo
lite group, and aloe vera group. The mice were anesthetized, and the dorsal hair was shaved over a 2×2 cm area using an
electric clipper. A second-degree burn wound model was created by exposing the shaved dorsal skin to hot air at 200°C for
10 seconds. A total of 100 μL of a bacterial metabolite suspension mixed with aloe vera at a 1:1 ratio was applied evenly
to the center of the wound. Wound images were captured daily, and wound areas were measured using ImageJ software.
Healing was evaluated on days 7, 14, and 21 of treatment and compared with the control group. The initial wound size was
defined as 100%, and wound closure percentages were calculated accordingly. Burn skin tissues from both experimen
tal and control groups were subjected to immunofluorescence staining for the vascular marker α-SMA (Thermo Fisher
Scientific). Statistical analysis: The differences between the control and experimental groups were analyzed using an
independent t-test using GraphPad Prism 10 software, and the mean, standard deviation, and statistical correlation were
calculated.
Results:The effect of the mixture of bacterial metabolites and aloe vera (Aloe barbadensis Miller) on the healing of burn
wounds in experimental mice was compared with the control group. The wound size at 7 days was 82.1% in the control
group and 83.7% in the experimental group (p<0.197), at 14 days was 62.6% in the control group and 58.1% in the exper
imental group (p<0.0001), at 21 days was 50.7% in the control group and 32.7% in the experimental group (p<0.0001).
No statistically significant difference in wound healing was observed between the groups at day 7; however, significant
differences were observed at days 14 and 21. Immunofluorescence analysis revealed uniform expression of α-SMA-pos
itive cells in the experimental group at 7, 14, and 21 days of treatment, whereas α-SMA expression was not detected in
the control group.
Conclusion:The combination of bacterial metabolites and aloe vera promotes wound healing in experimental mouse
burn wounds.
- Full text:202609221750183527005_Aloe_barbaden-Бактерийн_метаболит_ба_зуун_настын_sis_Miller_.pdf
