Determination of some virulence factors of Candida albicans cultured from samples of children with atopic dermatitis
- VernacularTitle:Атопийн арьсны үрэвсэлтэй хүүхдүүдийн шинжлэгдэхүүнээс өсгөвөрлөгдсөн Candida albicans-ийн хоруу чанарын зарим хүчин зүйлийг тодорхойлсон дүн
- Author:
Tunamal S
1
;
Erdenechimeg Ch
2
;
Enkhtur Ya
1
;
Otgonjargal B
3
Author Information
1. Department of Dermatology, School of Medicine, MNUMS
2. Outpatient Department, National Center for Dermatology
3. Department of Microbiology and Infection Prevention and Control, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Atopic dermatit, C.albicans, Biofilm, Phospholipase
- From:
Mongolian Journal of Health Sciences
2026;91(1):20-24
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:In atopic dermatitis, impairment of the skin barrier function leads to increased susceptibility to environ
mental particles and pollutants, thereby contributing to disease pathogenesis and complications, as well as to an increased
risk of fungal skin infections. In children with atopic dermatitis, infections caused by Candida species exacerbate the dis
ease and reduce the effectiveness of treatment. Candida species are common opportunistic pathogens colonizing the skin
and mucosal surfaces, and more than 200 species have been described. Among these, Candida albicans, C. glabrata, C.
parapsilosis, C. tropicalis, and C. krusei are most frequently isolated from the skin, mucosa, and oral cavity. In Mongolia,
studies culturing skin fungi and determining their metabolic activity and virulence factors are limited, which constituted
the rationale for the present study.
Aim:To determine virulence factors and selected biochemical activities of Candida species.
Materials and Methods:In this study, Candida species strains cultured from clinical samples of children with atopic der
matitis were used with research approval. The fungal strains preserved at –80°C were revived by culturing on Sabouraud
Chloramphenicol Agar and evaluated microscopically after Gram staining. Biofilm-forming activity was determined us
ing the microtiter plate method, in which cultures were grown in broth, optical density was measured using a microplate
reader, and values were calculated using a standard formula. Phospholipase activity was assessed using egg yolk agar
according to the method developed by Price et al.
Results:Of the total cultured isolates, 54.1% (n=33) demonstrated weak biofilm-forming activity (≤ODc / ODc < ~
≤2×ODc), while 32.79% (n=20) showed moderate biofilm-forming activity (2×ODc < ~ ≤4×ODc). Isolates exhibiting
strong biofilm-forming activity (>4×ODc) accounted for 13.1% (n =8) Regarding phospholipase activity, 50% of the
isolates showed strong activity, 30% showed moderate activity, 10% showed low activity, and 10% exhibited very weak
activity.
Conclusions:1. Virulent fungal strains capable of attaching to host cells are involved in the pathogenesis of atopic dermatitis.
2. Atopic dermatitis is shown to contain fungal strains that damage host cells and are involved in the process of inva
sion.
- Full text:20260922173025921403_Атопийн_арьсны_үрэвсэлтэй_хүүхдүүдийн_Candida_шинжлэгдэхүүнэ.pdf
