Bioactive Constituents of Anti-Impotence-4 and Its Effects in an Experimental Model of Erectile Dysfunction
- VernacularTitle:Анти-Импотенци-4 бэлдмэлийн биологийн идэвхт бодисын агууламж болон бэлгийн сулралын эмгэг загварт үзүүлсэн зарим үр дүн
- Author:
Shirchinmaa B
1
;
Chimedragchaa Ch
2
;
Anu A
2
;
Myadagbadam U
2
;
Batnairamdal Ch
3
Author Information
1. MNUMS, Mongolian International University of Medical Sciences
2. MNUMS, Mongolian National Hospital of Traditional Medicine
3. Institute of Traditional Medicine Technology
- Publication Type:Journal Article
- Keywords:
Reactive oxygen species, essential amino acids, phenolic compounds, erectile dysfunction
- From:
Mongolian Journal of Health Sciences
2026;91(1):12-19
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: Traditional medicine has long utilized herbal and animal-derived materials worldwide, and traditional
formulations for the management of male sexual dysfunction continue to be actively investigated. This study aimed to
investigate the chemical composition and antioxidant effects of Anti-Impotence-4, an herbal–animal formulation, in an
experimental model of chronic stress-induced erectile dysfunction (ED).
Aim:To investigate the bioactive compounds present in the plant- and animal-derived formulation Anti-Impotence-4 and
its effects against oxidative stress.
Materials and Methods:Fifty-eight male Wistar rats were randomly divided into six groups, including healthy control,
disease control, reference, and treatment groups. Chronic stress was induced once daily for 56 days using a random stress
protocol, as described by Peng Zou (2019). Oxidative stress parameters were determined using ELISA. The chemical
constituents of the methanolic extract of Anti-Impotence-4 were analyzed using ultra-performance liquid chromatogra
phy–mass spectrometry (UPLC–MS) in both positive and negative ionization modes.
Results: In an experimental model of erectile dysfunction induced by chronic stress, treatment with Anti-Impotence-4 led
to a dose-dependent attenuation of oxidative damage. Specifically, levels of the DNA oxidation marker 8-hydroxy-2′-de
oxyguanosine (8-OHdG) were reduced by 13.3–32.5% following administration of Anti-Impotence-4 at doses of 42,
84, and 210 mg/kg. Likewise, malondialdehyde (MDA) concentrations decreased by 7.9–23.7% at therapeutic doses
(p<0.05). In contrast, antioxidant defense mechanisms were markedly enhanced, as evidenced by significant increases
in superoxide dismutase (SOD) activity (34.1%, 38.1%, and 44.6%, respectively; p<0.05) and glutathione (GSH) levels,
which rose by 37.0–55.4%. Biological substances identified a total of 10 compounds, including six amino acids, flavo
noids, and iridoids, in the positive ion mode. In negative-ion mode, 18 bioactive compounds were detected, including
amino acids, carbohydrates, organic acids, fatty acids, flavonoids, and phenolic compounds.
Conclusion:Phytochemical analysis of the methanolic extract of the Anti-Impotence-4 formulation demonstrated the
presence of diverse bioactive constituents, including amino acids, purine derivatives, nucleosides, carbohydrates, organic
and fatty acids, flavonoids, phenolic compounds, and iridoids. In a chronic stress-induced erectile dysfunction model,
treatment with Anti-Impotence-4 significantly enhanced antioxidant defense by increasing superoxide dismutase and
glutathione levels, while markedly reducing oxidative stress markers such as 8-hydroxy-2′-deoxyguanosine and malond
ialdehe indicating significant antioxidant and protective effects.
- Full text:202609221726369582602_Анти-Импотенци-4_бэлдмэлийн_биологийн_идэвхт_бодисын_агуулам.pdf
