Pathogenic characteristics of measles virus genotype D8 in three cases in Daye City, Hubei Province in 2025
10.3969/j.issn.1006-2483.2026.05.007
- VernacularTitle:2025年湖北省大冶市三例麻疹病毒D8基因型病原学特征分析
- Author:
Ting ZHANG
1
;
Wenjing ZOU
1
;
Xianfeng TANG
1
;
Jing LI
1
Author Information
1. Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei 430079, China
- Publication Type:Journal Article
- Keywords:
Measles;
Genotype D8;
Homology;
Amino acid variation
- From:
Journal of Public Health and Preventive Medicine
2026;37(5):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the pathogenic characteristics of measles virus genotype D8 in three cases in Hubei Province. Methods An epidemiological investigation was conducted on three consecutive confirmed cases of measles in a city of Hubei Province within a week. Measles virus IgM antibody was detected by enzyme-linked immunosorbent assay, the measles virus nucleic acid was detected by real-time fluorescent quantitative polymerase chain, and the reverse transcription polymerase chain reaction was used to amplify the C-terminal 450-nucleotide sequence of the N gene. A phylogenetic tree of measles virus genotypes was constructed to compare the isolated strains with reference strains of measles from other regions, and the nucleotide homology and genotype were analyzed. Results The nucleotide homology of the N gene of three measles virus strains in Hubei Province in 2025 was 99.80%-100.00%, with one amino acid site difference. The N gene homology with the Swiss strain (MVs/Bern.CHE/19.23D8) and the Japanese strain (MVs/Tokyo.JPN/24.23/23124/D8) was 99.3% -99.7%, with one amino acid site difference. The nucleotide homology of N gene with the WHO D8 genotype reference strain (MVi/Manchester.GBR/30.94/D8) was 98.00%-98.20%, with six amino acid differences. The nucleotide homology of the N gene compared with the vaccine strain Shanghai-191 was 91.40%-91.60%, with 21 amino acid differences from the vaccine strain S191. Conclusion All the three measles virus strains in Hubei Province are of the genotype D8 and have a high homology. It is necessary to pay attention to imported cases of measles, and the etiological monitoring of measles virus should be continuously strengthened to provide a scientific basis for the elimination of measles.
