Banxia Xiexintang Containing Intestinal Absorption Solution Inhibits Gastric Cancer Cell Invasion and Migration by Modulating SDF1-CXCR4 Axis in TA-BMSCs
10.13422/j.cnki.syfjx.20261023
- VernacularTitle:半夏泻心汤含药肠吸收液通过干预TA-BMSCs中SDF1-CXCR4轴抑制胃癌细胞侵袭迁移
- Author:
Zhongbo ZHU
1
;
Wenying YANG
1
;
Jingjing WEI
1
;
Fangni LI
1
;
Lijuan SHI
1
;
Xiping LIU
1
Author Information
1. Gansu Provincial Key Laboratory of Exploration and Innovation Transformation of Chinese Medicine Prescriptions， Engineering Laboratory for New Traditional Chinese Medicine Product Development of Gansu Province， Gansu University of Chinese Medicine， Lanzhou 730000， China
- Publication Type:Journal Article
- Keywords:
Banxia Xiexintang;
drug-containing intestinal absorption solution;
tumor-associated bone marrow mesenchymal stem cells;
cell crosstalk
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(20):84-93
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore whether the intestinal absorption solution containing Banxia Xiexintang （BXT） can inhibit the invasion and migration of gastric cancer cells by interfering with the stromal cell-derived factor 1 （SDF1）-CXC chemokine receptor 4 （CXCR4） axis in tumor-associated bone marrow mesenchymal stem cells （TA-BMSCs）. MethodsThe intestinal absorption solution containing BXT was prepared， and the optimal intervention concentration and duration for MFC cells were determined through the cell counting kit-8 （CCK-8） assay. A co-culture system was established comprising TA-BMSCs conditioned medium （TA-BMSCs-CM） and gastric cancer MFC cells. The experiment was conducted with a blank control group， a TA-BMSCs-CM group， an SDF1 inhibitor （LY2510924） group， and intervention groups with varying concentrations （55%， 70%， 85%） of the intestinal absorption solution containing BXT. Cell proliferation was assessed by the CCK-8 assay. Horizontal and vertical cell migration were evaluated via scratch and Transwell assays， respectively. Cell invasion was examined by a Transwell assay with Matrigel. Cell apoptosis was detected by flow cytometry. The levels of factors such as SDF1， matrix metalloproteinase-9 （MMP-9）， and vascular endothelial growth factor A （VEGFA）， as well as the protein levels of macrophage migration inhibitory factor （MIF）， CXCR4， VEGFA， and MMP-9， were quantified by ELISA and Western blot， respectively. ResultsThe CCK-8 assay results indicated that compared with the 24 h intervention， the 48 h interventions with all concentrations of the intestinal absorption solution containing BXT increased the inhibition rate on MFC cells （P<0.01）. The half-maximal inhibitory concentration （IC50） of the intestinal absorption solution containing BXT at the time point of 48 h was 68.51%， and subsequent intervention concentrations were selected as 55%， 70%， and 85%. Compared with the TA-BMSCs-CM group， the intestinal absorption solution containing BXT （particularly at concentrations of 70% 85%） suppressed the proliferation， migration， and invasion of MFC cells， promoted the cell apoptosis （P<0.05， P<0.01）， decreased the levels of SDF1， MMP-9， and VEGFA， and downregulated the protein levels of MIF， CXCR4， VEGFA， and MMP-9 （P<0.05， P<0.01）. The inhibitory effects of the intestinal absorption solution containing BXT were comparable to or superior to those of the SDF1 inhibitor （P<0.01）. ConclusionThe intestinal absorption solution containing BXT can inhibit the invasion and migration of gastric cancer cells by interfering with the SDF1-CXCR4 axis in TA-BMSCs. The underlying mechanism may involve the regulation of the MIF/SDF1/CXCR4 signaling pathway and its downstream effector molecules.
