Study on changes and correlations of color，components and anti-tumor activity of processed products of Gelsemium elegans at different sand-frying durations
- VernacularTitle:不同砂炒时间钩吻炮制品的颜色、成分、抗肿瘤活性变化及相关性研究
- Author:
Hao WU
1
;
Lisong CHEN
1
;
Chenglong HUANG
1
;
Yueling WANG
1
;
Lirong CHEN
1
;
Wenyi WANG
2
;
Yinghao WANG
1
;
Desen LI
1
;
Shuisheng WU
1
;
Ying CHEN
1
Author Information
1. College of Pharmacy，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China
2. Science and Technology Innovation and Transformation Center，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China
- Publication Type:Journal Article
- Keywords:
sand-fried Gelsemium elegans;
chromatic value;
active components;
anti-tumor activity;
total alkaloids;
correlation analysis;
toxicity-attenuating and efficacy-preserving
- From:
China Pharmacy
2026;37(17):2258-2263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the changes and correlations of color， components and anti-tumor activity of Gelsemium elegans during sand-frying processing， so as to provide a reference for the toxicity-attenuating and efficacy-preserving processing as well as quality control of sand-fried G. elegans decoction pieces.METHODS Raw G.elegans and its processed products prepared by sand-frying for 15， 30， 75， 105 and 120 s were taken as test samples. The chromatic values， contents of total alkaloids and seven main active components （gelsemine， humantenine， koumine， gelsenicine， etc.） were determined. Their in vitro anti-tumor activity was investigated. The correlations between color and component contents， as well as between total alkaloid content and anti-tumor activity were analyzed.RESULTS The color of G.elegans samples generally darkened after sand-frying， and the total color difference （Δ E * ） of samples sand-fried for 75 s was greater than 20. At the initial stage of sand-frying （15-30 s）， the total alkaloid content in processed products decreased sharply compared with the raw material； at the later-stage of sand-frying （75-105 s）， the declining rate of total alkaloid content slowed down and tended to be stable. The contents of gelsenicine and humantenidine in processed products dropped drastically at 30 s of sand-frying， while the contents of koumine and sempervirine decreased substantially （by about 80%） after 75 s of sand-frying and then gradually tended to be stable. The anti-HepG2 cell proliferation activity of each processed product decreased with the extension of frying time. Correlation analysis demonstrated that color brightness and yellow-blue chromaticity were significantly positively correlated with the contents of total alkaloids and all determined active components except gelsemine （ P ＜0.05 or P ＜0.01）； red-green chromaticity was significantly positively correlated with the contents of koumine， sempervirine， gelsemivirine and humantenidine （ P ＜0.05 or P ＜0.01）； Δ E * was significantly negatively correlated with the contents of total alkaloids and all determined active components except gelsemine. Besides， total alkaloid content was significantly negatively correlated with the half maximal inhibitory concentration of the samples on HepG2 cells （ P ＜0.05 or P ＜0.01）.CONCLUSIONS With the prolongation of sand-frying time， the color of sand-fried G.elegans decoction pieces gradually turned brownish-brown， the contents of seven active components and total alkaloids decreased progressively， and its anti-HepG2 cell activity showed an overall downward trend. Sand-frying for 75 s serves as a critical turning-point for the above-mentioned changes.
