Establishment of content determination method for two specific C21‑steroid saponins in Marsdenia tenacissima decoction pieces
- VernacularTitle:通关藤饮片中两种特异性C21甾体皂苷含量测定方法的建立
- Author:
Qimin WANG
1
;
Qian JU
1
;
Liju FAN
2
;
Ying LI
2
;
Yinling MA
2
;
Zhanjun DONG
1
Author Information
1. Dept. of Pharmacy，Hebei General Hospital，Shijiazhuang 050051，China
2. Dept. of Pharmacy，Hebei General Hospital，Shijiazhuang 050051，China;Hebei Key Laboratory of Clinical Pharmacy，Shijiazhuang 050051，China
- Publication Type:Journal Article
- Keywords:
3- O - β -allopyranosyl-（1→4）- β -oleandropyranosyl-11- O -isobutyryl-12- O -acetyl tenacigenin B;
17 β -tenacigenin B;
content determination
- From:
China Pharmacy
2026;37(17):2254-2257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a content determination method for two specific C21‑steroid saponins namely 3- O - β -allopyranosyl-（1→4）- β -oleandropyranosyl-11- O -isobutyryl-12- O -acetyl tenacigenin B（compound T） and 17 β -tenacigenin B in Marsdenia tenacissima decoction pieces.METHODS Ultra‑high‑performance liquid chromatography‑tandem mass spectrometry （UPLC‑MS/MS） was adopted. An XSelect HSS T3 XP chromatographic column was used. Gradient elution was performed with 0.1% formic acid aqueous solution-methanol as the mobile phase. The flow rate was 0.3 mL/min， the injection volume was 5 μL， and the column temperature was 40 ℃. An electrospray ionization source was applied for mass spectrometry， and multiple reaction monitoring mode was used for positive‑ and negative‑ion acquisition.RESULTS The linear relationships of compound T and 17 β -tenacigenin B were good within the concentration ranges of 10-200 ng/mL and 20-200 ng/mL， with r values of 0.993 1 and 0.998 6， respectively； the RSD values of precision， repeatability， accuracy， and stability were all below 4.5% （ n ＝5-6）. Their contents in three batches of decoction pieces were 94.0-102.8 μg/g and 1 705.4-1 736.2 μg/g， respectively.CONCLUSIONS The established method can be applied for the content determination of the two specific C21‑steroid saponins mentioned above in M. tenacissima decoction pieces.
