Improvement effects of Yiqi yangyin huoxue formula on renal injury in IgA nephropathy rats and its relationship with NEAT1/RACK1/NLRP3 pathway
- VernacularTitle:益气养阴活血方对IgA肾病大鼠肾损伤的改善作用及其与NEAT1/RACK1/NLRP3通路的关系
- Author:
Xidong JIA
1
;
Chundong SONG
1
;
Xu WANG
1
;
Yifan TAO
2
Author Information
1. Nephropathy and Purpura Ward 2，Pediatric Hospital，The First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450099，China;School of Pediatrics，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Nephropathy and Purpura Ward 2，Pediatric Hospital，The First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450099，China
- Publication Type:Journal Article
- Keywords:
immunoglobulin A nephropathy;
inflammatory response;
renal injury;
NEAT1/RACK1/NLRP3 pathway
- From:
China Pharmacy
2026;37(17):2235-2240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the improving effect of Yiqi yangyin huoxue formula on renal injury in immunoglobulin A nephropathy （IgAN） rats， and explore its potential molecular mechanism based on nuclear-enriched abundant transcript 1 （NEAT1）/receptor of activated C kinase 1 （RACK1）/NOD-like receptor family pyrin domain containing 3 （NLRP3） pathway.METHODS SD rats were divided into blank group and model-establishing group. Rats in the model-establishing group were induced to establish IgAN model by combined treatment of bovine serum albumin， carbon tetrachloride and lipopolysaccharide. Rats with successful model establishment were randomly divided into model group， prednisone acetate group （6.25 mg/kg）， low-dose and high-dose Yiqi yangyin huoxue formula groups （11， 22 g/kg）. Each group was intragastrically administered corresponding drugs or normal saline once a day for 6 consecutive weeks. After the last administration， urinary red blood cell count， 24-hour urinary total protein （24 h-UTP）， serum creatinine （Cr）， blood urea nitrogen （BUN）， albumin （ALB）， alanine amino-transferase （ALT） and interleukin-1β （IL-1β） levels were detected. Pathomorphological changes of renal tissues and immunoglobulin A （IgA） deposition in glomerular mesangial region were observed. The mRNA expression of NEAT1， as well as mRNA and protein expression levels of RACK1 and NLRP3 in renal tissues were determined.RESULTS Compared with the blank group， the model group presented glomerular mesangial matrix hyperplasia accompanied by inflammatory cell infiltration， and extensive renal tubular dilation with focal atrophy. The levels of 24 h-UTP， urinary red blood cell count， serum IL-1β， Cr， BUN， ALT， mean fluorescence intensity of IgA deposition in glomerular mesangial area， mRNA expression level of NEAT1， mRNA and protein expression levels of RACK1 and NLRP3 in renal tissues were significantly increased （ P ＜0.05）， while serum ALB level was significantly decreased （ P ＜0.05）. Compared with the model group， the glomerular structure was basically restored and renal tubular injury was obviously alleviated in low-dose and high-dose Yiqi yangyin huoxue formula groups； all the above quantitative indicators were significantly reversed （ P ＜0.05）.CONCLUSIONS Yiqi yangyin huoxue formula can mitigate the inflammatory response and ameliorate renal injury in IgAN rats， and its mechanism may be related to the inhibition of NEAT1/RACK1/NLRP3 pathway activity.
