Influence and mechanism of curcumin on the biological behavior of oral squamous cell carcinoma cells based on network pharmacology and transcriptomics
10.12016/j.issn.2096-1456.202660095
- Author:
LIU Ji'an
1
;
XU Bo
1
;
ZHANG Ling
1
Author Information
1. Department of Oral and Maxillofacial-Head and Neck Oncology, the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University College of Stomatology; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Institute of Stomatology
- Publication Type:Journal Article
- Keywords:
curcumin;
oral squamous cell carcinoma;
PI3K/AKT signaling pathway;
lipid metabolism;
CAL-27 cells;
cell proliferation;
cell migration;
transcriptome analysis
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2026;34(9):871-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of curcumin on the biological behavior of oral squamous cell carcinoma (OSCC) cells and its potential molecular mechanisms, as well as to provide experimental evidence for the application of curcumin in the prevention and treatment of OSCC.
Methods:All animal experiments in this study were approved by the Institutional Animal Welfare and Ethics Committee and strictly adhered to relevant animal ethics guidelines. Potential intersecting targets of curcumin and OSCC were screened using TCMSP, SwissTargetPrediction, DrugBank, and GeneCards databases, followed by construction of a protein-protein interaction network and gene ontology(GO) /Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Scratch and clone formation experiments were conducted using CAL-27 and SCC-9 cells to detect the effect of curcumin on cell migration and proliferation ability. Combining RNA sequencing analysis of differentially expressed genes, and verifying the changes in mRNA and protein expression of key cellular molecules by curcumin through real-time fluorescence quantitative PCR and Western blot, respectively. Simultaneously establish a CAL-27 xenograft BALB/c nude mouse transplant tumor model and use immunohistochemistry to detect the effect of curcumin treatment on the expression of related proteins in the transplant tumor tissue.
Results:A total of 178 intersecting targets were identified, mainly enriched in the PI3K/AKT signaling pathway and lipid metabolism-related pathways. Curcumin significantly inhibited migration and colony formation of CAL-27 and SCC-9 cells in vitro. Transcriptomic analysis identified 828 differentially expressed genes enriched in lipid metabolism and PI3K/AKT pathways. Real-time fluorescence quantitative PCR showed downregulation of PIK3CA, CCND1, BCL2, and FASN, while western blot demonstrated decreased expression of p-AKT and FASN. In vivo, curcumin markedly suppressed tumor growth and reduced the expression of Ki-67, BCL2, and p-AKT in tumor tissues.
Conclusion:Curcumin may inhibit the migration and proliferation of OSCC cells by regulating the PI3K/AKT signaling pathway and the expression of lipid metabolism-related genes.
- Full text:2026091115191873936基于网络药理学和转录组学探讨姜黄素对口腔鳞状细胞癌细胞生物学行为的影响及机制.pdf
