Optimization of Characteristic Processing Technology for Polygalae Radix with Water Decoction of Coptidis Rhizoma from Jinling Medical School and Analysis of Its Chemical Constituent Variations Before and After Processing
10.13422/j.cnki.syfjx.20260967
- VernacularTitle:金陵医派特色炮制川连水炙远志的工艺优化及其炮制前后化学成分变化分析
- Author:
Yuanxin CHEN
1
;
Mengyang WANG
1
;
Fuqiong ZHOU
1
Author Information
1. Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine， Nanjing 210022， China
- Publication Type:Journal Article
- Keywords:
Jinling medical school;
characteristic processing;
Polygalae Radix processed with the water decoction of Coptidis Rhizoma;
process optimization;
chemical constituents
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(19):248-257
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo optimize the water extraction process of Coptidis Rhizoma （CR） and the processing of Polygalae Radix （PR） with the water decoction of CR， and to compare chemical constituents before and after processing. MethodsWith water volume， soaking time， decocting time， and decocting times as influencing factors， and the content of berberine， epiberberine， coptisine， jatrorrhizine， and palmatine and the yield of dry extract as evaluation indexes， the orthogonal test combined with multi-index comprehensive scoring method was employed to optimize the water extraction parameters of CR. With solid-liquid ratio， stir-frying temperature， and stir-frying time as influencing factors， and the content of tenuifolin， polygalaxanthone Ⅲ， and 3，6ʹ-disinapoyl sucrose and the yield of ethanol extract as evaluation indexes， the orthogonal test combined with multi-index comprehensive scoring method was applied to optimize the processing parameters of PR with the water decoction of CR. The differential chemical constituents of PR before and after processing were analyzed by ultra-performance liquid chromatography-quadrupole-Exactive Orbitrap-mass spectrometry （UPLC-Q Exactive Orbitrap MS）. ResultsThe optimal water extraction process of CR was as follows： Decocting for 3 times， 40 min each time， with 10 times the amount of water. The optimal processing technology of PR with the water decoction of CR was a solid-liquid ratio of 1∶1.5， a stir-frying temperature of 140 ℃， and stir-frying time of 20 min. A total of 31 differential components were identified before and after processing， including 17 components of PR and 15 components of CR. After processing， 14 new components such as coptisine， berberine， and palmatine emerged， and the content of arillanin A， glomeratose A， 3，6ʹ-disinapoyl sucrose， and ferulic acid was increased. The content of other intrinsic constituents of PR， such as onjisaponin D， polygalaxanthone Ⅺ， sibiricose A3， and tenuifoliside B， was generally decreased. ConclusionThe optimized characteristic processing technology of PR with the water decoction of CR is stable and feasible. Significant differences in chemical composition were observed between raw and processed PR. Processing with the water decoction of CR can effectively reduce the gastrointestinal irritant components originally present in PR while simultaneously introducing the bioactive constituents from CR. This study provides data support for the standardization research and mechanism elucidation of the ancient characteristic processing methods of the Jinling medical school.
