Experimental study on the method of establishing a pig left lung orthotopic transplantation model
- VernacularTitle:猪左肺原位移植模型构建方法的实验研究
- Author:
Heng ZHAO
1
;
Jinteng FENG
1
;
Shan GAO
1
;
Rui ZHAO
1
;
Hongyi WANG
1
;
Ye SUN
2
;
Yixing LI
1
;
Haotian BAI
1
;
Runyi TAO
1
;
Bin HE
1
;
Zhiyu WANG
1
;
Yanpeng ZHANG
1
;
Borui SUN
2
;
Shuo LI
1
;
Guangjian ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Lung transplantation; pig; donor lung procurement; transplantation protocol
- From: Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2026;33(08):1275-1281
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the method for establishing a pig left lung orthotopic transplantation model. Methods A total of 4 male Yorkshire pigs weighing (40.0±2.5) kg underwent surgical procedures, including anesthesia, tracheal intubation, donor lung procurement, and recipient lung transplantation. Histological morphology and blood gas analysis of the transplanted lungs were assessed 2 hours after reperfusion to evaluate the histological changes and function of the transplanted lungs. Results The pigs underwent left lung in situ transplantation, effectively simulating conditions relevant to human lung transplantation. Two hours after the transplantation, arterial blood gas analysis showed that arterial partial pressure of oxygen was 155.4-178.6 mm Hg, arterial partial pressure of carbon dioxide was 53.1-62.4 mm Hg, and the oxygenation index was 310.8-357.2 mm Hg. Hematoxylin and eosin staining indicated a low degree of pulmonary edema and minimal cellular infiltration. Conclusion The pig left lung orthotopic transplantation model possesses strong operability and stability. Researchers can replicate this model according to the described methods and further conduct basic research and explore clinical translational applications.
