Study on extraction technology of flavonoids from Potentilla discolor with deep eutectic solvents
- VernacularTitle:基于低共熔溶剂的翻白草中黄酮类成分的提取工艺研究
- Author:
Bin LIU
1
;
Chenyi YANG
2
;
Meiling SHAO
1
;
Liangyong HUANG
3
;
Yanguo GAO
1
;
Xueqin WANG
1
;
Guanghui LYU
1
;
Qian HU
1
;
Fang YE
1
Author Information
1. Dept.of Pharmacy，Taihe Hospital/Affiliated Hospital of Hubei University of Medicine，Hubei Shiyan 442000，China
2. School of Pharmacy，Hubei University of Medicine，Hubei Shiyan 442000，China
3. Dept.of Pharmacy，Taihe Hospital/Affiliated Hospital of Hubei University of Medicine，Hubei Shiyan 442000，China;School of Pharmacy，Hubei University of Medicine，Hubei Shiyan 442000，China
- Publication Type:Journal Article
- Keywords:
Potentilla discolor;
Flavonoids;
deep eutectic solvents;
Box-Behnken response surface methodology;
AHP;
CRITIC;
combined weighting
- From:
China Pharmacy
2026;37(16):2119-2123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the extraction process of flavonoids from Potentilla discolor .METHODS Deep eutectic solvents （DESs） were used as the extraction solvent to prepare test sample solutions. The contents of rutin， quercitrin， and kaempferol were determined by ultra-performance liquid chromatography. The analytic hierarchy process combined with the criteria importance through intercriteria correlation weighting method was employed to assign comprehensive weights to the contents of the three flavonoids， and calculate the comprehensive score. The optimal DESs were screened based on the comprehensive score.Mean while， using the comprehensive score as the response variable and the liquid-to-solid ratio， ultrasonic time， and urltrasonic temperature as the influencing factors， the extraction technology of flavonoids from P.discolor was optimized by Box-Behnken response surface methodology and subsequently validated.RESULTS The comprehensive weight coefficients of rutin， quercitrin， and kaempferol were 0.646 7， 0.207 5， and 0.145 8， respectively. The optimal DESs were choline chloride-lactic acid （molar ratio 1∶4） with a water content of 40%. The optimal extraction conditions were determined as follows： liquid-to-solid ratio of 25∶1， ultrasonic time of 40 min， and ultrasonic temperature of 50 ℃. The validation results showed that the average comprehensive score of three batches of samples was 99.28， with an RSD of 0.54% （ n ＝3）.CONCLUSIONS The optimal extraction technology of flavonoids from P. discolor based on DESs obtained in this study was stable and feasible.
