Effect of salvianolic acid B on the stored red blood cell quality and its underlying mechanism
10.13303/j.cjbt.issn.1004-549x.2026.08.003
- VernacularTitle:丹酚酸B对保存红细胞质量的影响及机制探究
- Author:
Chuan’ai CHEN
1
;
Haolong LI
1
;
Pengpeng AN
1
;
Yumiao YANG
1
;
Yang YU
1
Author Information
1. Department of Quality Management, Tianjin Blood Center, Tianjin 300110, China
- Publication Type:Journal Article
- Keywords:
salvianolic acid B (Sal B);
red blood cell storage;
oxidative stress;
storage lesion
- From:
Chinese Journal of Blood Transfusion
2026;39(8):1012-1017
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of salvianolic acid B (Sal B) on the quality of stored red blood cells (RBC) and to preliminarily explore its underlying mechanisms. Methods: Whole blood was collected from healthy adult volunteers, and suspended RBC were prepared and stored in MAP (mannitol-adenine-phosphate) solution. The RBC suspensions were divided into a control group and a Sal B group, with the latter supplemented with Sal B at a final concentration of 10 μM. All samples were mixed and stored at 2-6℃. RBC were collected on days 0, 7, 14, 21, 28, and 35 for evaluation. RBC damage was assessed through cell counting, morphological observation, osmotic fragility test, and hemolysis rate measurement. Cytosolic ROS levels and intracellular SOD activity were measured to evaluate oxidative stress. Results: Sal B helped maintain RBC morphology and osmotic stability, and reduced the generation of morphologically abnormal cells such as spherocytes and echinocytes. On days 28 and 35, the hemolysis rate in the Sal B group was significantly lower than that in the control group. Compared with the control group, the Sal B group exhibited decreased cytosolic ROS levels and increased intracellular SOD activity from day 14 onward. Conclusion: Sal B alleviates RBC storage lesion by reducing oxidative stress.
