Qiliqiangxin attenuates apoptosis of cardiomyocytes by modulating miR-21-5p/PTEN/PI3K/Akt pathway
10.12025/j.issn.1008-6358.2026.20260426
- VernacularTitle:芪苈强心通过调控miR-21-5p/PTEN/PI3K/Akt通路减轻心肌细胞凋亡
- Author:
Xiaoyu LIAN
1
;
Hui GONG
2
;
Xuyang WANG
3
Author Information
1. Department of Cardiology, Jinshan Hospital, Fudan University, Shanghai 201508, China;Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
2. Department of Cardiology, Jinshan Hospital, Fudan University, Shanghai 201508, China.
3. Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
- Publication Type:Originalarticle
- Keywords:
Qiliqiangxin;
oxidative stress;
cardiomyocyte;
miR-21-5p/PTEN/PI3K/Akt signaling pathway
- From:
Chinese Journal of Clinical Medicine
2026;33(4):592-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Qiliqiangxin protecting the cardiomyocytes against oxidative stress. Methods H9C2 cells were exposed to hydrogen peroxide (H2O2) to establish an oxidative stress-induced injury model and were divided into four groups: control group, H2O2 injury group, Qiliqiangxin rescue group (post-injury), and Qiliqiangxin prevention group (pre-treatment). Cell viability was assessed using cell counting Kit-8 (CCK-8) assay. Apoptosis rates were measured by flow cytometry. The expression of miR-21-5p was quantified using RT-qPCR. The levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), phosphatase and tensin homolog (PTEN), phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and phosphorylated Akt (p-Akt) were evaluated by Western blot analysis. Results H2O2 significantly reduced cell viability and increased apoptosis of H9C2 cells (P＜0.05). No statistically significant difference was observed in the Qiliqiangxin rescue group. However, Qiliqiangxin pre-treatment markedly enhanced cell viability and reduced apoptotic rates compared with the H2O2 injury group (P＜0.05). These protective effects were associated with downregulation of PTEN and upregulation of Bcl-2/Bax ratio, miR-21-5p, PI3K, and p-Akt/Akt ratio. Conclusions Qiliqiangxin can protect H9C2 cells against oxidative stress-induced injury, which may be associated with regulation of miR-21-5p/PTEN/PI3K/Akt signaling pathway.
