Study on HPLC fingerprint and multi-component determination of Ziziphus jujuba stir-fried with Coptis chinensis juice from Longsha Medical School
- VernacularTitle:龙砂医派黄连汁炒酸枣仁的HPLC指纹图谱和多成分含量测定方法研究
- Author:
Ping QIN
1
;
Yingying LU
2
;
Wenming ZHANG
2
;
Zifang FENG
2
;
Lihong GU
2
;
Chenjie XIA
2
;
Minmin HU
2
;
Xiaowei CHEN
2
;
Tulin LU
3
;
Yiting ZHANG
2
;
Zhenhua BIAN
2
Author Information
1. Dept. of Pharmacy，Wuxi Hospital of Traditional Chinese Medicine，Jiangsu Wuxi 214071，China;Branch of Longsha Medical School，Jiangsu Provincial Research Institute of TCM Academic Schools，Jiangsu Wuxi 214071，China
2. Dept. of Pharmacy，Wuxi Hospital of Traditional Chinese Medicine，Jiangsu Wuxi 214071，China
3. School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Ziziphus jujuba stir-fried with Coptis chinensis juice;
Longsha Medical School;
fingerprint chromatogram;
HPLC method;
processing medicinals with medicinals;
content determination;
quality control
- From:
China Pharmacy
2026;37(15):1974-1978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a high-performance liquid chromatography（HPLC） fingerprint and a multi-component content determination method for Ziziphus jujuba stir-fried with Coptis chinensis juice， a characteristic processed product of Longsha Medical School， and to provide a basis for the inheritance and clinical application of this distinctive variety. METHODS HPLC method was employed to establish the fingerprints of 12 batches of Z. jujuba stir-fried with C. chinensis juice. Similarity evaluation and common peak identification were subsequently carried out. Quantitative determination was performed on coclaurine， magnoflorine， coptisine hydrochloride， epiberberine， jatrorrhizine hydrochloride， spinosin， berberine hydrochloride， palmatine hydrochloride， 6"'-feruloylspinosin， and jujuboside A of samples. RESULTS The fingerprint similarities of all 12 batches of samples were higher than 0.960. A total of 17 common peaks were calibrated， among which 9 peaks were identified as coclaurine， magnoflorine， coptisine hydrochloride， epiberberine， jatrorrhizine hydrochloride， spinosin， berberine hydrochloride， palmatine hydrochloride， and 6"'-feruloylspinosin， respectively. The quantitative results showed that the mass fractions of coclaurine， magnoflorine， coptisine hydrochloride， epiberberine， jatrorrhizine hydrochloride， spinosin， berberine hydrochloride， palmatine hydrochloride， 6"'-feruloylspinosin， and jujuboside A in the 12 batches of samples were 0.044-0.091， 0.908-1.698， 0.325-0.549， 0.163-0.287， 0.103-0.172， 0.712-0.952， 1.185-1.843， 0.341-0.598， 0.228-0.405， and 0.050-0.091 mg/g， respectively. CONCLUSIONS The established HPLC fingerprint and multi-component determination method is stable and reliable， and can provide a basis for the quality standard formulation and clinical application of Z. jujuba stir-fried with C. chinensis juice.
