Correlation between NOX gene single nucleotide polymorphisms and susceptibility to noise-induced hearing loss
10.20001/j.issn.2095-2619.20260209
- VernacularTitle:NOX基因单核苷酸多态性与噪声性听力损失易感性的关联
- Author:
Lei SHAN
1
;
Ning ZHAO
;
Jie LIU
;
Xinyue HOU
;
Xiaoqin LI
;
Hai SU
Author Information
1. Xinjiang Uyghur Autonomous Region Third People's Hospital, Urumqi, Xinjiang 830000, China
- Publication Type:Journal Article
- Keywords:
Noise;
Hearing loss;
NOX;
Susceptibility;
Gene polymorphism
- From:
China Occupational Medicine
2026;53(1):58-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in the nicotinamide adenine dinucleotide phosphate-oxidase (NOX) 2 and NOX4 genes and susceptibility to noise-induced hearing loss (NIHL). Methods A total of 439 noise-exposed workers were recruited using a convenience sampling method. Based on pure-tone air conduction hearing threshold tests, participants were categorized into normal hearing, mild hearing loss, and moderate hearing loss groups, with 147, 154, 138 individuals, respectively. Peripheral venous blood samples of the workers were collected for DNA extraction. Genotyping of nine SNPs loci in NOX2 and NOX4 genes was performed using multiplex polymerase chain reaction, single-base extension, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results A total of seven of the nine SNPs loci conformed to Hardy-Weinberg equilibrium among the workers in the normal hearing group (all P>0.05). There was no significant difference in the genotype distribution or allele frequencies for these seven SNPs loci among workers in these three groups (all P>0.05). Ordinal logistic regression analysis showed that, after adjusting for confounders including age, sex, cumulative noise exposure, smoking, alcohol consumption, and earplug use, individuals carrying the GG genotype at rs200522762 had a lower risk of NIHL compared with those carrying the TT genotype under the codominant model (P<0.05). In the recessive model, individuals carrying the GG genotype at the rs200522762 locus had a lower risk than those carrying the GT+TT genotypes (P<0.05). Polymorphisms at the other six SNPs loci were not associated with NIHL risk (all P>0.05). Conclusion The NOX4 rs200522762 polymorphism is associated with susceptibility to NIHL.
