Analysis of the detection results of Beilong virus carried by small mammals in Haikou, China
10.3969/j.issn.1005-0507.2025.04.010
- VernacularTitle:海口市小型兽类携带北龙病毒检测结果分析
- Author:
Chong-cai WANG
1
;
Yan ZHANG
2
;
Su-ying LU
1
;
Jiao-jiao QIAN
3
;
Ya-meng XU
3
;
Jian-bin GUO
4
;
Wei-long TAN
3
Author Information
1. Hainan International Travel Healthcare Center(Haikou Customs Port Clinic, Haikou 570311, Hainan, China
2. General Hospital of Eastern Theater Command, Nanjing 210016, Jiangsu, China
3. Center for Disease Prevention and Control of Eastern Theater Command, Nanjing 210002, Jiangsu, China
4. Fuyin Hospital, Huzhou 313000, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
Rodent;
Paramyxovirus;
Beilong virus;
Epidemiology;
Haikou
- From:
Acta Parasitologica et Medica Entomologica Sinica
2025;32(4):260-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to understand the carriage status of Beilong virus(BeiV)in small mammals in Haikou, a city in Hainan Province, China, to provide a basis for the prevention and control of BeiV. Methods Following field sampling, a cDNA library was constructed from the tissues of small mammal specimens, including heart, liver, spleen, lung, kidney, brain, intestine, and muscle tissues. The presence of BeiV was detected using PCR, and some purified L gene fragments were subjected to Sanger sequencing. The complete genome sequences of members of the Paramyxoviridae virus family were obtained from the NCBI database using Python, using MAFFT(version 7)and MEGA-X for multiple sequence alignment and neighbor-joining method to construct a phylogenetic tree. Results A total of 38 rodent samples were collected, revealing a positive rate of 5.26%, with Rattus norvegicus exhibiting a positive rate of 7.41%. Evolutionary analysis indicated that BeiV carried by R. norvegicus in Haikou was similar to that found in R. norvegicus in Hong Kong,China, although notable genetic variations were also observed. Conclusions This study reports the first detection of BeiV in Haikou. Considering the reports of newly discovered Paramyxoviridae viruses in recent decades and the identification of BeiV in small mammals across more than a dozen provinces, it is evident that the distribution range of BeiV is much broader than previously assumed. This highlights the importance of enhancing rodent monitoring and control, as well as the screening and prevention of rodent-related pathogens.
