Morphological and molecular identification of Rhipicephalus sanguineus from dogs
10.3969/j.issn.1005-0507.2025.04.006
- VernacularTitle:犬源血红扇头蜱形态学及分子生物学鉴定
- Author:
Zhuang MIAO
1
;
Xi LI
2
;
Wen-jing LI
1
;
Fang-fang ZHOU
1
;
Shu-wen SUN
1
;
Tian-yin CHENG
1
;
Fen LI
1
Author Information
1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China
2. Changsha Academy of Agricultural Sciences, Changsha 410100, Hunan, China
- Publication Type:Journal Article
- Keywords:
Rhipicephalus sanguineus;
morphology;
16S rDNA;
cox1;
nad5;
phylogenetic tree
- From:
Acta Parasitologica et Medica Entomologica Sinica
2025;32(4):231-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aims of this study were to identify tick species infesting police dogs and explore their phylogenetic relationships. Methods Ticks were collected from a police dog base in Hunan Province and their diagnostic morphological characteristics and phylogenetic relationships were investigated using PCR targeting the 16S rDNA, cytochrome C oxidase subunit 1(cox1), and NADH dehydrogenase subunit V(nad5)genes. Results Adult ticks were oval shaped with a broad and short hexagonal basis capitulum. The females had a scutum occupying the first 1/3 of the body, a U-shaped genital groove, and a comma-shaped stigmal plate, and their coxae II to IV each had short and coarse outer spurs that gradually decreased in size according to the segmental order. Males had an elongated scutum, a comma-shaped stigmal plate, and a subtriangular adanal plate. The product sizes of the amplified 16S rDNA, cox1, and nad5 gene sequences from the ticks in this study were approximately 405,772, and 262 bp, respectively. The phylogenetic tree based on these sequences showed that this tick species clustered with Rhipicephalus sanguineus of other tropical lineages. Conclusions The tick species collected from police dogs in China is Rh. sanguineus, which belongs to the tropical lineage.
