Identification of 160 cases of difficult ABO blood group typing with forward typing as O group
10.13303/j.cjbt.issn.1004-549x.2026.07.015
- VernacularTitle:160例正定型为O型的疑难血型鉴定
- Author:
Lina WU
1
;
Lei MA
1
;
Shuangyu LI
1
;
Shiping AN
1
;
Jinhui XIE
1
Author Information
1. Tianjin Blood Center, Tianjin 300110, China
- Publication Type:Journal Article
- Keywords:
ABO blood group;
genotyping;
precise transfusion;
transfusion safety
- From:
Chinese Journal of Blood Transfusion
2026;39(7):917-921
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To accurately determine ABO blood groups through serological and molecular biological tests in patients whose forward typing was group O but who showed weak antibodies on one side of reverse typing, thereby ensuring precise transfusion. Methods: A total of 160 cases from 2023 to 2025 at Tianjin Blood Center, initially screened as O type with weak antibodies or missing antibodies on one side of reverse typing, were collected. Serological tests absorption-elution tests, and molecular biological tests were performed to accurately identify their ABO blood groups and ensure transfusion safety. Results: Among the 160 patients with forward typing as O type and weak antibodies on one side of reverse typing, 92 were identified as O type, 46 as ABO subtypes already named and recorded by ISBT (mainly including 18 cases of Ael02, 8 cases of AW37, and 9 cases of Bel03), 12 suspected as new alleles not yet recorded by ISBT (among which 5 were suspected to carry novel alleles that have not been recorded or reported in the literature), and 10 were suspected as weakened A/B antigens. Conclusion: In clinical transfusion, attention should be paid to cases with ABO forward-reverse discrepancy, where forward typing is O type and reverse typing shows weak antibodies on one side. These cases may involve ABO subtypes, and further testin is necessary to accurately determine the ABO blood group, achieve precise transfusion, and avoid blood wastage.
