Study on preparation of universal platelet-rich plasma
10.13303/j.cjbt.issn.1004-549x.2026.07.004
- VernacularTitle:通用型富血小板血浆制备方法的研究
- Author:
Lu WANG
1
;
Qiong WU
2
;
Xinyue YAO
1
;
Pingping MAO
1
;
Yi YANG
1
;
Kaiyun LUO
1
;
Na LI
1
;
Shujun WANG
1
Author Information
1. Department of Blood Transfusion, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
2. Department of Scientific Research and Training, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
- Publication Type:Journal Article
- Keywords:
platelet-rich plasma;
titer of blood group antibodies;
microcolumn gel anti-human globulin test;
growth factor
- From:
Chinese Journal of Blood Transfusion
2026;39(7):840-845
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To improve the efficiency of patient treatment in routine, emergency, and mass casualty scenarios (both peacetime and wartime), eliminate reliance on blood type compatibility, and promote the productization of platelet-rich plasma (PRP), this study investigated the optimal mixing ratio for developing a universal PRP formulation. Methods: PRP was prepared from donors with blood types O, A, B, and AB. Samples were mixed in varying proportions, and the optimal ratio was determined based on the trend of anti-A and anti-B antibody titers in the mixed PRP. Concentrations of the growth factors PDGF-AA, PDGF-BB, EGF, TGF-β1, and VEGF were measured by enzyme-linked immunosorbent assay (ELISA) before and after mixing. Results: A mixing ratio of A∶B∶AB=6∶2.5∶24 resulted in no detectable agglutination with either A or B cells in the microcolumn gel system, indicating the absence of immunological reactivity and confirming universal compatibility. Importantly, the levels of all measured growth factors remained unchanged following mixing, suggesting preservation of biological activity. Conclusion: These findings suggest that universal PRP can be prepared by neutralizing blood type antibodies through antigen-antibody reactions.
