POTENTIAL OF CHITOSAN BLACK SOLDIER FLIES (Hermetia Illucens) PUPAE ON POST-EXTRACTION WOUND HEALING PROCESS

Renie Kumala Dewi; Renie Kumala Dewi; Sri Oktawati; Asdar Gani; Eko Suhartono; Nurlinda Hamrun; Shahida Mohd Said; Beta Widya Oktiani; Zakiah Husada Noor; Yunita Marwah

Return

POTENTIAL OF CHITOSAN BLACK SOLDIER FLIES (Hermetia Illucens) PUPAE ON POST-EXTRACTION WOUND HEALING PROCESS

Author: Renie Kumala Dewi ¹ ; Renie Kumala Dewi ² ; Sri Oktawati ³ ; Asdar Gani ³ ; Eko Suhartono ⁴ ; Nurlinda Hamrun ⁵ ; Shahida Mohd Said ⁶ ; Beta Widya Oktiani ⁷ ; Zakiah Husada Noor ⁸ ; Yunita Marwah ⁹
Author Information

1. Doctoral Program of Dentistry, Faculty of Dentistry, Hasanuddin University, 90245, Makassar, Indonesia
2. Department of Pediatric Dentistry, Faculty of Dentistry, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
3. Department of Periodontology, Faculty of Dentistry, Hasanuddin University, 90245, Makassar, Indonesia
4. Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
5. Department of Oral Biology, Faculty of Dental Medicine, Hasanuddin University, 90245, Makassar, Indonesia
6. Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur, Malaysia
7. Department of Periodontology, Faculty of Dentistry, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
8. Student of the Faculty of Dentistry, Lambung Mangkurat University, 70122, Banjarmasin, Indonesia
9. Resident of a Periodontology Program, Faculty of Dentistry, Airlangga University, Indonesia
Publication Type:Journal Article
Keywords: Black Soldier Fly Pupae; Chitosan; Fibroblasts; Macrophages; Wound Healing
From: Journal of University of Malaya Medical Centre 2024;27(Special Issue):349-358
CountryMalaysia
Language:English
Abstract: POTENTIAL OF CHITOSAN BLACK SOLDIER FLIES (Hermetia Illucens) PUPAE ON POST-EXTRACTION WOUND HEALING PROCESS:Chitosan can accelerate the wound healing process by increasing inflammatory cells, such as macrophages, and increases fibroblast proliferation after tooth extraction. The Black Soldier Fly (BSF) pupae containing 35% chitin, which can be processed into chitosan and was found to have potential benefits in post-extraction wound healing. This study aimed to assess the potential of chitosan BSF pupae by monitoring the presence of macrophages and fibroblasts after tooth extraction. Chitosan BSF pupae was made into a gel preparation. Eighteen male Cavia cobaya were divided into control and treatment groups (n = 9 each). BSF pupae chitosan gel was applied to the post-extraction tooth socket in the treatment group (CBSF), while none was applied to the control group (CC). The samples were decapitated on the 2nd, 3rd, and 5th days by cutting the mandibular bone for histopathological examination, which was carried out by measuring the number of macrophages and fibroblasts through microscopic observation and data analysis. There an increase in macrophages evident in CBSF group than in CC group on the 2nd and 3rd days, while a decrease was seen on the 5th day of CBSF group than CC group. There was an increase in fibroblasts evident in CBSF group than in CC group on the 2nd, 3rd, and 5th days. Kruskal Wallis test indicated a significant difference in macrophages between the control and the treatment group (p = 0.006), and the One-way ANOVA test showed a significant difference in fibroblasts between both groups (p = 0.000). In conclusion, the application of chitosan BSF pupae accelerated the post-extraction wound healing process in Cavia cobaya by modulating the number of macrophages and increasing the number of fibroblasts.
Full text:2026081116502307413POTENTIAL OF CHITOSAN.pdf