Comparative analysis of hematopoietic reconstitution after different stem cell infusions in a murine model of bone marrow acute radiation syndrome
10.13491/j.issn.1004-714X.2026.03.015
- VernacularTitle:不同干细胞输注对骨髓型急性放射病小鼠造血重建的比较分析
- Author:
Xuan LIU
1
;
Yixi HOU
1
;
Qianqian XIAO
1
;
Fang ZHOU
1
Author Information
1. The 960th Hospital of the Joint Logistics Support Force of the People's Liberation Army of China, Jinan 250031, China.
- Publication Type:OriginalArticles
- Keywords:
Acute radiation syndrome;
Umbilical cord blood transplantation;
Haploidentical transplantation;
Mesenchymal stem cells;
Hematopoietic reconstitution
- From:
Chinese Journal of Radiological Health
2026;35(3):407-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the therapeutic efficacy and identify differences between haploidentical hematopoietic stem cell transplantation combined with mesenchymal stem cells (Haplo-MSC) and umbilical cord blood hematopoietic stem cell transplantation combined with mesenchymal stem cells (UCB-MSC) in mice with bone marrow acute radiation syndrome (ARS). Methods Eight-to ten-week-old CB6F1 mice were exposed to 8 Gy of 60Co γ-ray irradiation to establish a bone marrow-type ARS model.The mice were then randomly assigned to three groups: the Haplo-MSC group (haploidentical hematopoietic stem cells combined with MSCs), the UCB-MSC group (umbilical cord blood stem cells combined with MSCs), and a saline control group. The survival rate, peripheral blood cell counts, pathological changes in target organs, colony formation of bone marrow cells, and graft-versus-host disease (GVHD) were observed. Results All mice in the saline control group died within 14 days, with a median survival time of 8.9 days. The 30 day survival rate was 65% in the Haplo-MSC group and 60% in the UCB-MSC group. The Log-rank test showed no significant difference in survival time between the two transplantation groups (P=0.561). At 28 days post-transplantation, no significant differences were observed in WBC, PLT, RBC, or Hb counts between the two groups (P=0.151, 0.733, 0.856, and 0.300, respectively). Pathological changes in bone marrow cells showed that bone marrow failure occurred later in the UCB-MSC group than in the Haplo-MSC group. In contrast, the Haplo-MSC group exhibited faster hematopoietic stem cell engraftment. Furthermore, the numbers of CFU-E, CFU-GM, and CFU-GEMM differed significantly between the two groups (P<0.05). Conclusion Both the Haplo-MSC and UCB-MSC regimens promoted hematopoietic engraftment and reconstitution in mice with lethal-dose radiation-induced bone marrow ARS. However, the onset of bone marrow failure was delayed in mice treated with the UCB-MSC regimen. In contrast, mice treated with the Haplo-MSC regimen showed faster engraftment.
