Identification and content determination of active components in Shugantongluo capsules
10.12206/j.issn.2097-2024.202405048
- VernacularTitle:疏肝通络胶囊有效成分的鉴别和含量测定
- Author:
Bo DAI
1
;
Fang WANG
1
;
Fangchu XU
1
;
Min ZHOU
1
;
Hailong YUAN
1
;
Zhihui YANG
1
Author Information
1. Department of Pharmacy, Air Force Medical Center, Air Force Medical University, Beijing 100142, China.
- Publication Type:Originalarticles
- Keywords:
Shugantongluo capsules;
TLC;
HPLC;
Saikosaponin-a
- From:
Journal of Pharmaceutical Practice and Service
2026;44(7):358-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of thin layer chromatography （TLC） for identification and content quantitative determination of Shugantongluo capsules, and provide methodological reference for the quality control of Shugantongluo capsules. Methods Bupleuriradix, polygala tenuifolia yuanzhi, and acorus tatarinowii rhizoma were identified by TLC qualitatively. The content of Saikosaponin-a was determined by HPLC method. The chromatography was performed on Venusil XBP C18（L） （4.6 mm×150 mm, 5 μm） with a stable temperature of 30℃. The mobile phase in isocratic elution consisted of acetonitrile-water （40∶60, V/V） at the flow rate of 1.0 ml/min with an injection volume of 20 μl. The detection wavelength was set at 210 nm. Results Clear spots were obtained with good separation in TLC identification which was highly specific. The Saikosaponin-a at the range of 25.00-500.0 μg/ml was linear with peak area （r=0.999 9）, the average recovery rate was 97.00%, and RSD was 2.60%. Conclusion These methods was easy to operate with accurate results, with good specificity and reproducibility, which could be uesd for the quality control for Shugantongluo capsules.
