Study on quality standards of Liuwei Runfu oil recipe
10.12206/j.issn.2097-2024.202406033
- VernacularTitle:六味润肤油摩方的质量标准研究
- Author:
Yue HU
1
;
Yi WANG
2
;
Minghua MA
3
;
Fulun LI
2
;
Hua NIAN
2
;
Jianyong ZHU
2
Author Information
1. Department of Pharmacy Research, 1b.Department of Pharmacy, 1c.Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Pharmacy Research, 1b.Department of Pharmacy, 1c.Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
2. Department of Pharmacy Research, 1b.Department of Pharmacy, 1c.Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
3. Yangpu Hospital of Tongji University, Shanghai 200090, China.
- Publication Type:Originalarticles
- Keywords:
Liuwei Runfu oil recipe;
quality standard;
high-performance liquid chromatography;
thin layer chromatography
- From:
Journal of Pharmaceutical Practice and Service
2026;44(7):353-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a standardized quality criterion for the formula of the Liuwei Runfu oil recipe. Methods The qualitative identification of the components, such as Cannabis sativa, Ligusticum chuanxiong, Angelica sinensis, Coix lacryma-jobi, and Glycyrrhiza uralensis, was conducted by thin layer chromatography （TLC）. The concentration of Senkyunolide A, ligustilide, and linoleic acid was determined by high-performance liquid chromatography （HPLC）. Results The TLC identification results demonstrated excellent differentiation and high selectivity, without any disruption from the negative samples. The reference standard for Senkyunolide A exhibited excellent linearity in the range of 25 to 300 µg （r=0.9999）, with an average recovery rate of 102.3% and a relative standard deviation （RSD） of 2.3%. The ligustilide reference standard exhibited strong linearity between the concentrations of 0.6875 and 8.25 µg （r=0.9993）, with an average recovery rate of 100.1% and a relative standard deviation （RSD） of 2.8%. The linoleic acid reference standard demonstrated strong linear correlation within the range of 2.8125 to 33.75 µg （r=0.9990）, with an average spiking recovery rate of 102.7% and a relative standard deviation （RSD） of 1.7%. Conclusion The established method with strong specificity was simple，accurate，reliable，which coulod provide a reference for the quality control of the Liuwei Runfu oil recipe.
