Effects of Yishen tongluo formula on high glucose-induced injury in renal tubular epithelial cells and its impact on the SIRT3-AMPK/PGC-1α/CPT1A pathway
- VernacularTitle:益肾通络方改善高糖诱导的肾小管上皮细胞损伤及其对SIRT3-AMPK/PGC-1α/CPT1A通路的影响
- Author:
Fengyi LI
1
;
Jian ZHONG
2
Author Information
1. Faculty of Chinese Medicine Science，Guangxi University of Chinese Medicine，Nanning 530222 China
2. Dept. of Nephrology，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，China
- Publication Type:Journal Article
- Keywords:
Yishen tongluo formula;
renal tubular epithelial cells;
high glucose;
energy metabolism;
oxidative stress;
SIRT3-AMPK/PGC-1α/CPT1A signaling pathway
- From:
China Pharmacy
2026;37(13):1710-1715
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the protective effect of Yishen tongluo formula on high glucose-induced injury in renal tubular epithelial cells and its impact on the silence information regulator 3 （SIRT3）-adenosine monophosphate-activated protein kinase （AMPK）/peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α）/carnitine palmitoyltransferase 1A （CPT1A） signaling pathway. METHODS Human renal tubular epithelial cells HK-2 were used as the subject. The cells were divided into a normal glucose group （NC group）， a high glucose group （HG group）， a high glucose+low-dose Yishen tongluo formula group [HG+L group， 0.5 mg/mL （calculated by crude drugs）]， and a high glucose+high-dose Yishen tongluo formula group [HG+H group， 1.0 mg/mL （calculated by crude drugs）]. Except for the NC group， cells in all other groups were cultured under high glucose conditions （30 mmol/L）， and cells in the HG+L and HG+H groups were simultaneously treated with the corresponding mass concentrations of medicinal liquid. Cell viability was assessed at 0， 24， and 48 h post-incubation， and reactive oxygen species （ROS） level， adenosine triphosphate （ATP） content， and the expression of molecules related to the SIRT3-AMPK/PGC-1α/CPT1A signaling pathway were measured after 48 h of incubation. RESULTS Compared with the NC group， the HG group showed significantly reduced cell viabilities （at 24 and 48 h） and ATP content， along with significantly elevated ROS levels （ P ＜0.05）. Additionally， the protein expressions of SIRT3， PGC-1α and CPT1A， as well as the mRNA expressions of PGC-1α and CPT1A， and the phosphorylation level of AMPK protein were all significantly downregulated or decreased（ P ＜0.05）. Compared with the HG group， the aforementioned indicators were significantly improved in both HG+L and HG+H groups， with the improvement being more pronounced in the HG+H group （ P ＜0.05）. CONCLUSIONS The Yishen tongluo formula can improve high-glucose-induced damage to renal tubular epithelial cells； its action may be related to activating the SIRT3-AMPK/PGC-1α/CPT1A signaling pathway， improving mitochondrial energy metabolism， and alleviating oxidative stress.
