Downregulated vascular endothelial growth factor A as a biomarker of immunosenescence in benign prostatic hyperplasia
10.12483/j.issn.1009-8291.2026.01.013
- VernacularTitle:下调的血管内皮生长因子A是良性前列腺增生免疫衰老的标志物
- Author:
Junduo WANG
1
;
Jun ZHU
1
;
Yanbo CHEN
1
;
Meng GU
1
;
Jiatai HE
2
;
Bin XU
1
;
Qi CHEN
1
Author Information
1. Department of Urology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200011
2. Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430021, China
- Publication Type:Journal Article
- Keywords:
benign prostatic hyperplasia;
immunosenescence;
vascular endothelial growth factor;
vascular endothelial growth factor A;
vascular endothelial growth factor receptor;
VEGF pathway;
placental growth factor
- From:
Journal of Modern Urology
2026;31(1):67-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify potential immunosenescence markers in benign prostatic hyperplasia(BPH)and to explore BPH-related senescent phenotypes. Methods Three transcriptome datasets and one single-cell RNA-sequencing(scRNA-seq)dataset for BPH and normal prostate tissues were obtained from the Gene Expression Omnibus(GEO)database. The GSE7307 and GSE119195 were combined to analyze differentially expressed genes(DEGs). Enrichment analysis and immune infiltration analysis were used to explore aging-related changes in the immune microenvironment. Weighted gene coexpression network analysis(WGCNA)was applied to identify immune-related genes in GSE132714. ScRNA-seq data were employed to investigate the transcriptomic landscape, luminal cell heterogeneity, and aging-related alterations in BPH through cell-cell communication and pseudotime analysis. Finally, the results were validated with in vitro experiments. Results A total of 1202 DEGs and 627 immune-related genes were identified in BPH and normal prostate tissues. Enrichment analysis indicated that the vascular endothelial growth factor(VEGF)signaling pathway exhibited a downregulated aging phenotype in BPH. In scRNA-seq analysis, 9 cell types were identified. The VEGF signaling pathway in BPH exhibited an aging-related expression pattern, characterized by decreased vascular endothelial growth factor A(VEGFA)in luminal cells. Conclusion Our study identified VEGFA as a marker of immunosenescence in BPH, with changes of the VEGF pathway. Meanwhile, low expression of VEGFA, coupled with high expression of placental growth factor(PlGF)and vascular endothelial growth factor receptor(VEGFR), represented a senescent phenotype of the prostate.
