Genetic diversity analysis of Aedes albopictus populations in Shandong Province using mitochondrial mtDNA-COⅠ gene sequences
10.3969/j.issn.1005-0507.2026.01.005
- VernacularTitle:基于线粒体COⅠ基因分析山东省白纹伊蚊种群遗传多样性
- Author:
Fan-jin MENG
1
;
Yong LIU
2
;
Huan HUANG
1
;
Wei-bo MA
3
;
Yi-fan WU
3
;
Wei-long TAN
1
Author Information
1. Nanjing Medical University, Nanjing 211166, Jiangsu, China; Eastern Theater Command Center for Disease Control and Prevention, Nanjing 210002, Jiangsu, China
2. Northern Theater Command Center for Disease Control and Prevention, Shenyang 110034, Liaoning, China
3. Eastern Theater Command Center for Disease Control and Prevention, Nanjing 210002, Jiangsu, China
- Publication Type:Journal Article
- Keywords:
Aedes albopictus;
Mitochondrial cytochrome C oxidase subunit I;
Population genetic structure;
Genetic diversity
- From:
Acta Parasitologica et Medica Entomologica Sinica
2026;33(1):31-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the genetic diversity, differentiation, and population structure of Aedes albopictus across different geographical regions in Shandong Province, and to explore the relationship between genetic diversity and geographical distribution. Methods Between July and August 2024, ten Ae. albopictus populations were sampled from seven cities in Shandong Province. Genomic DNA was extracted from individual mosquitoes, and the mitochondrial cytochrome c oxidase subunit I(COⅠ)gene was amplified using PCR and sequenced. The obtained sequences were verified using BLAST and analyzed with MAFFT, MEGA 11, DnaSP v6.12, Arlequin 3.5, PopART 1.7, STRUCTURE 2.3.4 and the R packages adegenet and vegan to assess genetic diversity and population structure. Results A total of 229 COⅠ sequences(662 bp) was obtained, revealing ten variable sites with no insertions or deletions. The overall base composition showed an AT bias of 67.7%. Haplotype analysis identified 11 haplotypes, with Hap2 being the dominant and most widely distributed haplotype across all populations. Neutrality tests showed significant population expansion only in the Rizhao population. Mantel testing result revealed that geographical distance does not significantly impede gene flow. Overall, genetic differentiation among populations was low, indicating frequent gene flow. When combined with additional samples from Shanghai, Fujian, Guangdong, Yunnan, Hainan and Guangxi, STRUCTURE, UPGMA and DAPC analyses revealed two primary genetic clusters of Ae. albopictus. Conclusions Ae. albopictus populations in Shandong Province exhibit frequent gene flow, low genetic differentiation, and relatively low overall genetic diversity. However, the Rizhao population showed signs of recent expansion, highlighting the need for enhanced surveillance and targeted control measures in this area.
