Identification and infection rate of densovirus in Culex pipiens pallens in Beijing in 2023
10.3969/j.issn.1005-0507.2026.01.004
- VernacularTitle:2023年北京市淡色库蚊中浓核病毒的鉴定及感染率研究
- Author:
Xiu-yan XU
1
;
Ting YAN
2
;
Si-jie ZHU
3
;
Jing LI
1
;
Mei-de LIU
1
;
Hong-jiang ZHANG
1
;
Ting LIU
1
;
Qiu-hong LI
1
;
Xiao-jie ZHOU
1
;
Ying TONG
1
;
Yong ZHANG
4
Author Information
1. Institute of Disinfection and Vector Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
2. Institute of Disinfection and Vector Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China; School of Public Health, Capital Medical University, Beijing 100069, China
3. Disinfection Department, Beijing Shunyi District Center for Disease Prevention and Control, Beijing 101300, China
4. School of Public Health, Capital Medical University, Beijing 100069, China; Institute of Disinfection and Vector Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
- Publication Type:Journal Article
- Keywords:
Parvovirus;
Brevihamaparvovirus;
Culex pipiens pallens;
Densovirus;
Infection rate
- From:
Acta Parasitologica et Medica Entomologica Sinica
2026;33(1):25-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study conducted molecular biological identification of the viruses carried by Culex pipiens pallens specimens collected in Shunyi District, Beijing in 2023, and observed the changes in the infection rate of the viruses carried by Cx. pipiens pallens at different collection times. Methods Cx. pipiens pallens were collected using carbon dioxide mosquito traps. The mosquito samples were ground in batches and analyzed by molecular biology technologies. The virus infection rate at different collection times was analyzed statistically. Results 17 strains of Culex pipiens pallens densovirus(CppDNV)were identified from Cx. pipiens pallens samples collected in Shunyi District, Beijing, in 2023. The nucleotide sequence analysis of the virus genome coding region showed that CppDNV was a single-stranded DNA virus with a total length of 3 335 nt, encoding 2 non-structural proteins(NS1, NS2)and 1 capsid protein(VP). The nucleotide(amino acid)sequence lengths of the three proteins were 2 376 nt(791 aa),1 092 nt(363 aa)and 1 071 nt(356 aa), respectively. Phylogenetic analysis showed that CppDNV was located in genus Brevihamaparvovirus. Statistical analysis showed no significant difference in infection rates across collection times(χ2=4.429, P=0.194). Conclusions CppDNV was identified in Cx. pipiens pallens in Beijing, and it was stably maintained in this natural population.
