Genetic differentiation of Anopheles sinensis (Diptera: Culicidae) based on COⅠ and ITS2 sequences
10.3969/j.issn.1005-0507.2026.02.003
- VernacularTitle:基于COI和ITS2序列的中华按蚊遗传分化研究
- Author:
Gang WANG
1
;
Chun-juan SHI
1
;
Dan KONG
1
;
Xuan ZHU
1
;
Ji HE
2
;
Ming-hui ZHAO
3
;
Chong-cai WANG
4
Author Information
1. Hangzhou International Travel HealthCare Center, Hangzhou 310012, Zhejiang, China
2. Xiamen International Travel HealthCare Center, Xiamen 361013, Fujian, China
3. Nanchang International Travel HealthCare Center, Nanchang 330026, Jiangxi, China
4. Haikou International Travel HealthCare Center, Haikou 528051, Hainan, China
- Publication Type:Journal Article
- Keywords:
Anopheles sinensis;
Genetic differentiation;
COⅠ gene;
ITS2 sequence
- From:
Acta Parasitologica et Medica Entomologica Sinica
2026;33(2):90-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective Exploring genetic differences among geographic strains of Anopheles sinensis in China. Methods Based on the mitochondrial cytochrome oxidase subunit I(COⅠ )and ribosomal the second internal transcribed spacer(ITS2), genetic distance analyses were conducted on An. sinensis samples collected nationwide. Results Genetic differentiation was observed within An. sinensis geographic strains, primarily reflected in the genetic distances of COⅠ sequences, with some distances between samples exceeding those between closely related species. However, ITS2 sequences showed relatively low levels of differentiation within geographic strains. No significant separation was observed, and gene exchange exhibited a random pattern among geographic strains. Conclusions Significant differences in genetic distance were observed among An. sinensis samples, along with evidence of interspecific continuity among sibling species. For the identification of closely related species using wild-collected Anopheles mosquitoes samples, reliance solely on commonly used barcodes such as COⅠ and ITS2 may be insufficient. Additional molecular markers, including COⅡ and D3 gene fragments, should also be considered. Caution should be exercised when using COⅠ genes to identify cryptic species, especially within Anopheles mosquitoes.
