Standardization and validation of analytical methods for Inula helenium L. root
- VernacularTitle:Өндөр зоосон цэцэг (Inula helenium L.) Ургамлын үндсийн стандартчилал ба шинжилгээний аргын баталгаажилт
- Author:
Oyun-Erdene Purevsuren
1
;
Sugarmaa Battulga
2
;
Batdorj Davjid
2
;
Nomin Jagar
2
;
Battulga Borbaatar
2
;
Badral Duger
3
;
Lkhaasuren Ryenchindorj
2
;
Khurelbaatar Luvsan
2
;
Badamtsetseg Soyollkham
2
Author Information
1. Drug Research Institute, Monos Group, Ulaanbaatar, Mongolia; Mongolian University of Pharmaceutical Sciences;
2. Drug Research Institute, Monos Group, Ulaanbaatar, Mongolia
3. Mongolian University of Pharmaceutical Sciences
- Publication Type:Journal Article
- Keywords:
Inula helenium L.;
polysaccharide;
inulin;
sesquiterpene lactone;
alantolactone;
isoalantolactone;
standardization;
HPLC
- From:
Mongolian Pharmacy and Pharmacology
2026;29(2):55-61
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:Inula helenium L. is a medicinal plant widely used in traditional Mongolian and
Tibetan medicine. Its roots and rhizomes contain active compounds, including sesquiterpene
lactones (alantolactone and isoalantolactone), inulin, flavonoids, and essential oils, with anti-inflammatory, antimicrobial, and immunomodulatory properties. This study evaluates the
quality of Inula helenium L. roots and quantifies their key active compounds in accordance with
pharmacopoeial standards.
Methods:The roots of Inula helenium L. cultivated in the Medicinal Plant Botanical Garden of
Monos Group were used as raw material. Physicochemical parameters, including moisture, total
ash, acid-insoluble ash, and extractive values, were determined according to pharmacopoeial
methods. Qualitative analysis was carried out using color reactions for polysaccharides and
thin-layer chromatography for the identification of sesquiterpene lactones. Quantitative analysis
was performed using spectrophotometry for total polysaccharides and high-performance liquid
chromatography (HPLC) for the determination of alantolactone and isoalantolactone.
Conclusion:The physicochemical parameters of Inula helenium L. root were standardized, with
total inulin content determined as 47.13±0.45%, alantolactone as 1.41±0.02%, and isoalantolactone
as 1.33±0.02%. Analytical methods for inulin, alantolactone, and isoalantolactone were validated
according to ICH Q2(R2) guidelines, confirming the possibility of updating the MNS standard or
developing a manufacturer’s pharmacopoeial monograph based on these validated methods.
- Full text:2026080202063199321MPPJ-2026-29(2)-55-61.pdf
