Honokiol inhibits the malignant progression of gastric cancer cells by regulating the ATF4/CHOP/TRIB3 pathway
10.19405/j.cnki.issn1000–1492.2026.05 005
- VernacularTitle:和厚朴酚通过调节ATF4/CHOP/TRIB3通路抑制胃癌细胞恶性进程
- Author:
Kaihong DAI
1
;
Xianhui WEN
1
;
Yun HUANG
1
;
Sixi WEI
1
;
Hai HUANG
1
Author Information
1. Center for Clinical Laboratories， The Affiliated Hospital of Guizhou Medical University，Guiyang 550004
- Publication Type:Journal Article
- Keywords:
honokiol;
gastric cancer;
endoplasmic reticulum stress;
ATF4/CHOP/TRIB3 signaling pathway;
apoptosis;
targeting
- From:
Acta Universitatis Medicinalis Anhui
2026;61(5):827-835
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of honokiol on proliferation， apoptosis， migration， and invasion of gastric cancer cells and its underlying mechanistic. MethodsHuman gastric cancer cell lines HGC-27 and AGS were treated with Honokiol at concentrations of 0， 15， and 25 μmol/L. CCK-8 assays were conducted to determine the half maximal inhibitory concentration （IC50） for both cell lines. Cell viability， proliferation， migration， and invasion capabilities were assessed using CCK-8， colony formation， wound healing， Transwell migration and Transwell invasion assays. Apoptosis rates were measured via flow cytometry. Western blot analysis examined proteins related to proliferation， apoptosis， migration， invasion， and the endoplasmic reticulum stress pathway ATF4-CHOP-TRIB3. ResultsCompared with the control group， treatment with 15 and 25 μmol/L Honokiol significantly reduced the proliferation， colony formation， migration， and invasion capabilities of the two gastric cancer cell lines， while significantly increasing the apoptosis rate （P<0.05）. Additionally， compared to the control group， the protein expression levels of neural cadherin（N-cadherin）， Vimentin， proliferating cell nuclear antigen（PCNA）， and B-cell lymphoma/leukemia-2 protein（Bcl-2）decreased in the two gastric cancer cell lines after treatment with 15 and 25 μmol/L Honokiol， while the protein expression levels of epithelial cadherin（E-cadherin）， Bcl-2-associated X protein（Bax）， activating transcription factor 4（ATF4）， endoplasmic reticulum stress-related protein（CHOP）， and tribbles homolog 3（TRIB3）increased（P<0.05）. ConclusionHonokiol promotes apoptosis and inhibits proliferation， migration， and invasion of gastric cancer HGC-27 and AGS cells by regulating the ERS signaling pathway ATF4/CHOP/TRIB3.
