Role of exosomal miR-320c in gingerol-mediated defense against Staphylococcus aureus infection
10.19405/j.cnki.issn1000–1492.2026.05.001
- VernacularTitle:外泌体内miR-320c在姜酚抵御金葡菌感染中的作用
- Author:
Zhencai XING
1
;
Mengxue XU
1
;
Xiang KONG
1
;
Jinghan SUN
1
;
Zhen MA
1
;
Yu GAO
1
;
Siyuan DU
1
;
Hong ZHENG
1
;
Yakun LIU
1
Author Information
1. Department of Pathophysiology， School of Basic Medical Sciences，Anhui Medical University， Hefei 230032
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
gingerol;
extracellular vesicles;
endothelial barrier;
VE-cadherin
- From:
Acta Universitatis Medicinalis Anhui
2026;61(5):795-802
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the inhibitory effect of gingerol， an active component of ginger， on Staphylococcus aureus （S. aureus） infection， and to preliminarily explore its mechanism related to extracellular vesicles （EVs）. MethodsS.aureus infection models were established in human umbilical vein endothelial cell （HUVECs）， Vero E6 cells， and C57BL/6 mice. Experimental groups included control， infection， and gingerol-treated groups. Bacterial load and VE-cadherin protein expression were detected using immunofluorescence and Western blot. EVs were isolated by size exclusion chromatography and characterized by transmission electron microscopy and nanoparticle tracking analysis. miRNA sequencing was performed on EVs. ResultsGingerol treatment significantly reduced the bacterial load in both in vitro and in vivo infection models and upregulated VE-cadherin expression. miRNA sequencing of EVs revealed that S. aureus infection upregulated the expression of hsa-miR-320c， while gingerol treatment reversed this abnormal expression. Bioinformatic analysis further predicted that the target genes of hsa-miR-320c were significantly enriched in cell junction-related pathways. ConclusionGingerol exhibits clear antibacterial and host-protective effects， by regulating hsa-miR-320c in EVs to maintain endothelial barrier integrity.
