hsa_circ_0034762 contributes to cisplatin resistance in tongue squamous cell carcinoma CAL27-res cells via miR-17-3p-mediated regulation of autophagy
DOI：10.3872/j.issn.1007-385X.2026.06.007
- VernacularTitle:hsa_circ_0034762通过miR-17-3p调控自噬参与舌鳞状细胞癌CAL27-res细胞顺铂耐药
- Author:
FANG Songcheng1
1
,
2
,
3
;
GAO Xiaolin2
1
,
2
,
3
;
SUN Wenhao3
1
,
2
,
3
;
XIE Shule3
1
,
2
,
3
;
CHENG Huilin3
1
,
2
,
3
Author Information
1. Department of Stomatology, Dongguan Tungwah Hospital, Dongguan 523110, Guangdong, China;
2. Department of Stomatology, Haizhu District Stomatological Hospital of Guangzhou, Guangzhou 510220, Guangdong, China;
3. Department of Stomatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510030, Guangdong, China
- Publication Type:Journal Article
- Keywords:
舌鳞状细胞癌；环状RNA；miR-17-3p；化疗耐药；自噬
- From:
Chinese Journal of Cancer Biotherapy
2026;33(6):651-660
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探究环状RNA（circRNA）调控舌鳞状细胞癌（TSCC）顺铂（CDDP）耐药的机制。方法：本研究以TSCC化疗敏感CAL27细胞与耐药CAL27-res细胞为研究对象，RT-qPCR检测两种细胞内hsa_circ_0034762的表达量差异，流式细胞术检测细胞凋亡率，Western blotting检测自噬相关蛋白p62及LC3-Ⅱ/LC3-Ⅰ比值，透射电镜观察自噬小体的数量，双萤光素酶报告实验检测hsa_circ_0034762与miR-17-3p的结合关系，荧光原位杂交实验检测二者在细胞中的共定位，敲低hsa_circ_0034762联合抑制miR-17-3p进行功能回复实验。结果：相比亲本化疗敏感细胞，TSCC耐药细胞CAL27-res自噬水平显著提升，表现为自噬小体数量增加、LC3-Ⅱ/LC3-Ⅰ比值升高及p62蛋白表达降低。hsa_circ_0034762在TSCC耐药组织及CAL27-res细胞中高表达。敲低hsa_circ_0034762后，CAL27-res细胞凋亡率升高，自噬小体数量减少，p62蛋白表达增加，LC3-Ⅱ/LC3-Ⅰ比值降低（均P < 0.05）。双萤光素酶报告实验与荧光原位杂交实验发现hsa_circ_0034762与miR-17-3p存在靶向结合作用。抑制miR-17-3p可部分逆转hsa_circ_0034762敲低所致的促凋亡及抑制自噬效应。结论：hsa_circ_0034762在TSCC耐药细胞及组织中高表达。敲低hsa_circ_0034762可能通过miR-17-3p抑制细胞自噬并促进细胞凋亡，从而降低TSCC细胞的化疗耐药性。
- Full text:2026070710533671511202606007.pdf
