Preliminary study on the characterization of exosomes in cryoprecipitate
10.13303/j.cjbt.issn.1004-549x.2026.06.003
- VernacularTitle:冷沉淀中细胞外泌体表征的研究
- Author:
Yingyi TANG
1
;
Dawei CHEN
1
;
Ailing TAN
1
;
Xintong LI
1
;
Yujian LIU
1
;
Huibin ZHONG
1
Author Information
1. Guangzhou Blood Center, Institute of Blood Transfusion and Hematology of Guangzhou Medical University, The Key Medical Laboratory of Guangzhou, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
cryoprecipitate;
exosome;
specific protein;
exosome characterization
- From:
Chinese Journal of Blood Transfusion
2026;39(6):718-723
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the types and levels of exosomes in cryoprecipitate from donors of different blood types, genders, and age groups, and to preliminarily characterize their specific proteins. Methods: Exosomes were extracted from cryoprecipitate using the polymer precipitation method. The exosomes were identified by particle size analysis, transmission electron microscopy, and Western blot. Finally, the specific proteins CD63, CD81, CD9, Cytochrome C, Syntenin-1, and VLA-4 of exosomes were characterized using Luminex. Results: The purified exosomes exhibited a double-membrane vesicle structure under microscopy, appearing saucer-shaped or as hemispheres with a concave side. They specifically expressed the CD63 and TSG101 proteins, while the endoplasmic reticulum protein Calnexin was not expressed. The particle sizes(nm) of exosomes from blood types A, B, O, and AB were 119.6±47.5, 120.5±47.4, 133.3±54.1 and 103.1±35.3, respectively. Taking the exosomal specific protein CD63 as an example, its expression levels(pg/mL) in cryoprecipitate from female and male donors were 128.22±63.52 and 117.39±40.84, respectively; the expression(pg/mL) levels in the 20-40 and 40-60 age groups were 114.20±37.28 and 131.41±64.98, respectively; and the expression levels(pg/mL) in the four blood types A, B, O, and AB were 109.58±37.79, 127.45±44.46, 131.89±40.59, and 122.29±78.68, respectively. The expression levels of CD63 in exosomes from different blood types, genders, and age groups were similar, with no statistically significant differences (all P>0.05). The distribution of the other five exosomal-specific proteins was also comparable among the normal blood donors. Conclusion: The expression levels of exosomal-specific proteins in cryoprecipitate from normal blood donors are not associated with blood type, gender, and age group.
