Apolipoprotein A-I Enhances Eosinophil Activation and Survival in Response to Fungal Ligands
- Author:
Wanjin KIM
1
;
Jiwon LEE
;
Chae Yeong KO
;
Hye Li KIM
;
Jemin KIM
;
Han Kyoung CHO
;
Juhan YOON
;
Chang Ook PARK
Author Information
1. Department of Dermatology, Severance Hospital and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea
- Publication Type:Original Article
- From:Korean Journal of Dermatology
2025;63(6):155-164
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Apolipoprotein A-I (ApoA-I), the major protein component of high-density lipoprotein, is increasingly recognized for its immunomodulatory roles beyond lipid transport, including antimicrobial and anti-inflammatory functions. Although ApoA-I can modulate immune cell activity, its effect on eosinophils remains poorly understood.
Objective:This study aimed to determine whether ApoA-I could modulate human eosinophil activation and survival during fungal stimulation and to elucidate the underlying mechanisms.
Methods:Human eosinophils were isolated and stimulated with Alternaria alternata or β-glucan in the presence or absence of recombinant ApoA-I. Eosinophil degranulation was assessed by measuring eosinophil-derived neurotoxin (EDN) release using an enzyme-linked immunosorbent assay. Surface CD11b expression and cell viability were evaluated using flow cytometry. Dose-response experiments and comparisons with apolipoprotein A-II (ApoA-II) and interleukin-5 (IL-5) were also conducted.
Results:ApoA-I markedly increased EDN secretion and upregulated CD11b expression upon fungal stimulation. It also improved eosinophil viability, with effects comparable to those of IL-5. These responses were specific to ApoA-I, as ApoA-II did not elicit similar effects.
Conclusion:ApoA-I enhanced eosinophil activation and survival following fungal stimulation. These findings underscore the context-dependent immunomodulatory role of ApoA-I and its potential as a regulator of antifungal immunity.
