Molecular characterisation and histopathological analysis of canine distemper virus in a Malayan tiger (Panthera tigris jacksoni)
- Author:
Muhammad Farris MOHD SADALI
1
;
Abdul Razak MARIATULQABTIAH
;
Annas SALLEH
;
Nurul Izzati Uda ZAHLI
;
Tengku Rinalfi Putra Tengku AZIZAN
;
Hafandi AHMAD
;
Mohd Arifin KADERI
;
Khor Kuan HUA
;
Ridhwan Abdul WAHAB
;
Ahmad Lutfi ABDULLAH
;
Millawati GANI
;
Farina MUSTAFFA-KAMAL
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2026;27(1):e4-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This study aims to describe histological manifestations and characterise the CDV strain in the tiger to determine its molecular epidemiology and postulate viral pathogenicity.
Methods:Histopathology characterisation of the infected tissues were performed through haematoxylin and eosin and immunohistochemistry staining, respectively. We conducted virus isolation and titration in Chinese hamster ovarian expressing the dog signalling lymphocytic activation molecule (CHO-SLAM) cells. Reverse transcription polymerase chain reaction was performed to confirm the presence of CDV RNA, nucleotide and amino acid sequence analyses for comprehensive characterisation of the CDV strain.
Results:Notable histopathological changes were observed primarily in the brain, lung, liver, kidney, spleen, and stomach, with viral antigens localised in the lung, liver, kidney, and stomach tissues. CDV-induced cell cytopathic effects yielding 4.27 × 10 6 TCID 50 /mL were observed at 48 h post-inoculation in CHO-SLAM cells. Phylogenetic analysis suggested that the virus originated from the Asia-1 clade. Notably, 549H and 519I mutations in the hemagglutinin protein were observed, indicating adaptation to a non-canid wildlife species.
Conclusions:and Relevance: Overall, this study enhances our understanding of the molecular characterisation and evolutionary dynamics of the CDV strain present in the Malayan tiger and serves as a benchmark for developing effective preventative measures to protect Malayan tigers and mitigate their risk of extinction.
