Comparative analysis of ERK and CREB activity in normal and cryptorchid testes of bulls
- Author:
Changjin YUN
1
;
Sohee JEONG
;
Yongbin CHO
;
Sang-Ik PARK
;
In Sik SHIN
;
Jun-Gyu PARK
;
Changjong MOON
;
Sohi KANG
;
Joong-Sun KIM
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2025;26(6):e83-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This study aimed to examine morphological changes and changes in the extracellular signal-regulated kinase (ERK) and cyclic AMP response element-binding protein (CREB) activity in the testes of bulls with cryptorchidism.
Methods:Testes from the 18-month-old unilateral cryptorchid bulls, including the descended (normal) and undescended (cryptorchid) testes from each animal, were analyzed through gross examination, histology, immunohistochemistry (Ki-67, phosphorylated p-ERK, p-CREB), and Western blotting.
Results:Cryptorchid testes were smaller and exhibited reduced epithelial thickness, although seminiferous tubule area remained unchanged. Expression of Ki-67, p-ERK, and p-CREB was significantly decreased, indicating impaired cell proliferation and signaling.Total ERK and CREB protein levels were unchanged, but phosphorylation levels were notably reduced in cryptorchid samples.
Conclusions:and Relevance: Our findings demonstrate that cryptorchidism alters testicular morphology and disrupts key signaling pathways, particularly those involved in cell growth and function. Such alterations may contribute to infertility and highlight the importance of early diagnosis and intervention in the context of reproductive management in livestock.
