Baicalin modulates metabolic and inflammatory proteins and attenuates neuronal damage in a rat model of ischemic stroke
- Author:
Hyun-Kyoung SON
1
;
Dong-Ju PARK
;
Hun-Hwan KIM
;
Ju-Bin KANG
;
Phil-Ok KOH
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2025;26(6):e87-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:To identify proteins regulated by baicalin in a rat model of ischemic stroke.
Methods:Adult male Sprague Dawley rats underwent middle cerebral artery occlusion (MCAO) or sham surgery and received intraperitoneal baicalin (100 mg/kg) or vehicle 1 h before surgery. Neurobehavioral assessments were performed 24 h after MCAO, and cerebral cortex tissues were collected. Cortical damage was evaluated using 2,3,5-triphenyltetrazolium chloride staining and hematoxylin-eosin staining. Protein expression changes between groups were assessed by liquid chromatography-tandem mass spectrometry, and selected targets were validated by reverse transcription-polymerase chain reaction.
Results:MCAO induced marked neurological deficits, infarction, and histopathological damage, all of which were significantly attenuated by baicalin treatment. MCAO decreased adenylate cyclase type 1 and solute carrier family 25 member 12 levels, and baicalin mitigated these reductions. Baicalin also reduced MCAO-induced increases in C-reactive protein and apolipoprotein C-II, as well as alpha-1 microglobulin, murinoglobulin, and hemoglobin subunit B, proteins associated with inflammation, lipid metabolism, mitochondrial function, and cellular injury.
Conclusions:and Relevance: Baicalin exerts neuroprotective effects in cerebral ischemia by modulating proteins involved in energy metabolism, myelination, and neuroinflammation.These findings support baicalin as a promising therapeutic candidate in experimental stroke models.
