A Case of Canine Colorectal Carcinoma In Situ with Regulatory T Cell Infiltration
10.17555/jvc.2024.41.4.207
- Author:
Yunhee JOUNG
1
;
Jiwoong YOON
;
Dong Ju LEE
;
Woo-Jin SONG
;
Jongtae CHEONG
;
Hyunjung PARK
;
Young-min YUN
;
Gee Euhn CHOI
;
Myung-Chul KIM
Author Information
1. Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea
- Publication Type:Case Report
- From:
Journal of Veterinary Clinics
2024;41(4):207-214
- CountryRepublic of Korea
- Language:English
-
Abstract:
An adult castrated male dog was presented with persistent hematochezia. Digital rectal examination and endoscopy found multiple colorectal masses. Complete blood count and serum biochemical results were within the reference interval. Fine needle aspirate of the masses indicated a diagnosis of inflamed polyps with a primary differential of malignancy. Histopathologic examination using endoscopy-guided incisional biopsy of the masses revealed an inflamed neoplasm with ossification. A colectomy was performed to remove the tumor. Subsequent histopathologic examination of the surgically resected masses resulted in a diagnosis of colorectal carcinoma in situ (CiS) with immune infiltrates, which were subject to immunohistochemical and flow cytometric immunophenotyping. The immunohistochemistry confirmed intraepithelial CD3 + T cells within CiS. The flow cytometric analysis indicated tumor-infiltrating CD4 + T, CD8 + T, and CD11b + myeloid subsets. The flow cytometric analysis of circulating and tumor-infiltrating leukocytes demonstrated a preferential expansion of CD25 + FOXP3 + regulatory T cells within CiS. To the author’s knowledge, this is the first report to show clinical evidence emphasizing the immunogenicity and immune-suppressive environment of canine colorectal CiS. Our case will be valuable in providing a rationale for basic research that dissects the immune environment for canine colorectal cancers for the future development of cancer immunotherapy.
