Microbiota composition of human milk based on storage and processing methods in Korea
- Author:
Changin OH
1
;
Insoo KANG
;
Ben KANG
;
Dae Yong YI
Author Information
- Publication Type:Research Paper
- From:Nutrition Research and Practice 2026;20(1):89-100
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES:The microbiota present in human milk (HM) plays a crucial role in infant health and development. Maintaining it during storage and thawing is vital for preserving its beneficial properties; however, the effects of storage period, temperature, and thawing conditions on HM are not well understood.
SUBJECTS/METHODS:HM samples from lactating mothers were collected and stored under different temperature conditions (room temperature [RT], refrigeration, and −20°C) for different durations (1 week and 4 weeks), and thawed using different methods (RT, bottle warmer, and microwave), followed by analysis of their microbiota.
RESULTS:Storing HM samples at RT for a week and at −20°C for four weeks resulted in a significant reduction in microbiota diversity, whereas the other treatments did not lead to a significant change in beta diversity. In the microbiota distance analysis, HM samples stored at −20°C for a week showed the highest similarity to the initial microbiota compared to those after one week of storage at RT or in a refrigerator. Microwave thawing after 1 week of freezing resulted in the greatest alteration in the initial microbiota composition, whereas thawing at RT or with a bottle warmer showed comparable microbial profiles.
CONCLUSION:The most suitable method for storing and thawing HM while preserving the microbiota is to store it at −20°C for up to one week and thaw it using a bottle warmer.
