Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus
10.1016/j.phrp.2015.11.004
- Author:
Abolfazl FATEH
;
Mohammadreza AGHASADEGHI
;
Seyed D. SIADAT
;
Farzam VAZIRI
;
Farzin SADEGHI
;
Roohollah FATEH
;
Hossein KEYVANI
;
Alireza H. TASBITI
;
Shamsi YARI
;
Angila ATAEI-PIRKOOH
;
Seyed H. MONAVARI
- Publication Type:Original Article
- From:
Osong Public Health and Research Perspectives
2016;7(2):83-89
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objectives:This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.
Methods:A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.
Results:The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).
Conclusion:Screening by ARMS – PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.
