Traditional and Modern Cell Culture in Virus Diagnosis
10.1016/j.phrp.2015.11.011
- Author:
Ali HEMATIAN
1
;
Nourkhoda SADEGHIFARD
;
Reza MOHEBI
;
Morovat TAHERIKALANI
;
Abbas NASROLAHI
;
Mansour AMRAEI
;
Sobhan GHAFOURIAN
Author Information
1. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
- Publication Type:Review Article
- From:
Osong Public Health and Research Perspectives
2016;7(2):77-82
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification of viruses. In addition, the advantages and disadvantages of both traditional and modern cell culture methods for diagnosis of each type of virus are discussed. Detection of viruses by the novel cell culture methods is considered more accurate and sensitive. However, there is a need to include some more accurate methods such as molecular methods in cell culture for precise identification of viruses.
