Systematic evaluation and optimization of test volume in flow imaging microscopy
10.13200/j.cnki.cjb.004713
- VernacularTitle:流动成像法检测体积的系统评估与优选
- Author:
Sha GUO
- Publication Type:Journal Article
- Keywords:
Insoluble particles;
Safety;
Flow imaging microscopy(FIM);
Quality control;
Test volume
- From:
Chinese Journal of Biologicals
2026;39(06):704-714+722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of test volume of flow imaging microscopy(FIM) on detection performance,and to determine a FIM test volume with practical application value.Methods Two FIM platforms from different manufac-turers were employed to systematically evaluate the performance of four test volumes across three particle concentration levels,two sample matrices,and three particle types,in order to clarify the effect of test volume on FIM measurement results.Results The findings showed that within the practically operable test volume range,differences in measurement outcomes among different test volumes were generally small,indicating good methodological stability.Notably,a test volume of appro-ximately 200 μL provided the most favorable balance among repeatability,measurement stability,and test representative-ness,yielding the lowest relative standard deviation(RSD) values.Conclusion A test volume of 200 μL appears to be a suitable choice that balances repeatability and representativeness,although further optimization should be conducted based on sample-specific characteristics and related factors.This study provides valuable preliminary evidence to support the standardization and procedural development of FIM-based methods for insoluble particle testing.
